人肝细胞生长因子重组腺病毒枕大池注入对动脉粥样硬化大鼠脑组织IL-6、TNF-α表达的影响

发布时间：2018-03-21 09:41

本文选题：人肝细胞生长因子重组腺病毒　切入点：动脉粥样硬化　出处：《山东医药》2017年43期 　论文类型：期刊论文

【摘要】：目的观察人肝细胞生长因子重组腺病毒(Ad-HGF)枕大池注入对动脉粥样硬化大鼠脑组织IL-6、TNF-α表达的影响。方法 60只15月龄SD雄性大鼠,随机抽取50只采用肾动脉狭窄术及高脂饲料喂养法制作动脉粥样硬化模型,造模成功大鼠20只;将造模成功大鼠随机分为动脉粥样硬化组、Ad-HGF组,各10只;另10只大鼠为对照组。对照组、动脉粥样硬化组大鼠通过枕大池注入生理盐水(10μL),Ad-HGF组大鼠通过枕大池注入Ad-HGF(10μL,滴度5×109pfu/100μL);之后第10天处死大鼠,用免疫组织化学法检测大鼠脑组织中的IL-6及TNF-α,用image-proplus软件对IL-6及TNF-α在大鼠脑组织中的表达进行半定量分析,同时采用酶联免疫吸附法检测大鼠脑组织中的IL-6及TNF-α。结果免疫组织化学法检测显示,对照组、动脉粥样硬化组、Ad-HGF组大鼠脑组织中的IL-6相对表达量分别为0.216 0±0.068 1、0.331 0±0.043 3、0.174 9±0.010 7,TNF-α相对表达量分别为0.078 6±0.011 2、0.255 1±0.045 8、0.054 4±0.016 6;与对照组相比,动脉粥样硬化组IL-6、TNF-α的表达增加(P均0.05);与动脉粥样硬化组相比,Ad-HGF组IL-6、TNF-α的表达减少(P均0.05)。酶联免疫吸附法检测显示,对照组、动脉粥样硬化组、Ad-HGF组大鼠脑组织中的IL-6表达量分别为(50.415±6.690)、(108.302±5.695)、(66.062±9.550)pg/m L,TNF-α表达量分别为(83.836±9.442)、(209.425±15.520)、(85.996±8.445)pg/m L;与对照组相比,动脉粥样硬化组IL-6、TNF-α的表达增加(P均0.05);与动脉粥样硬化组相比,Ad-HGF组IL-6、TNF-α的表达减少(P均0.05)。结论动脉粥样硬化大鼠脑组织IL-6、TNF-α的表达增加,Ad-HGF可以减少动脉粥样硬化大鼠脑组织IL-6、TNF-α的表达,即抑制动脉粥样硬化大鼠脑组织炎性反应。
[Abstract]:Objective to observe the effect of human hepatocyte growth factor recombinant adenovirus (Ad-HGF-) on the expression of IL-6 TNF- 伪 in the brain of atherosclerotic rats. Fifty rats were randomly divided into atherosclerosis group (n = 10) and atherosclerosis group (n = 10), which were randomly divided into atherosclerotic group (n = 10) and atherosclerotic group (n = 10). The other 10 rats were used as the control group. The rats in the atherosclerosis group were injected with 10 渭 L saline through the cistern of the occipital cistern. The rats in the Ad-HGF group were injected with Ad-HGF(10 渭 L through the cistern of the occipital cistern, the titer was 5 脳 10 9 pfur / 100 渭 L, and the rats were killed on the 10th day after that. The expression of IL-6 and TNF- 伪 in rat brain was detected by immunohistochemical method, and the expression of IL-6 and TNF- 伪 in rat brain was semi-quantitatively analyzed by image-proplus software. IL-6 and TNF- 伪 in rat brain were detected by enzyme-linked immunosorbent assay (Elisa). The relative expression of IL-6 was 0.216 0 卤0.068 1 卤0.331 0 卤0.043 3 + 0.174 9 卤0.010 7 TNF- 伪 in the brain of rats in the atherosclerotic group and 0.078 6 卤0.011 2 卤0.255 1 卤0.045 8 卤0.054 4 卤0.016 6, respectively, compared with the control group, the relative expression of TNF- 伪 was 0.078 6 卤0.011 2 卤0.255 1 卤0.045 8 卤0.054 4 卤0.016 6, respectively. The expression of IL-6 TNF- 伪 increased in atherosclerosis group and decreased in Ad-HGF group compared with atherosclerosis group. The expression of IL-6 TNF- 伪 in Ad-HGF group was lower than that in atherosclerotic group. The results of Elisa showed that the expression of IL-6 TNF- 伪 in the control group was lower than that in the control group. The expression of IL-6 in the brain of rats in the atherosclerotic group was 50.415 卤6.690, 108.302 卤5.695 and 66.062 卤9.550 pg / m ~ (-1) TNF- 伪, respectively (83.836 卤9.442), 85.996 卤8.445pg / mL, and 85.996 卤8.445pg / mL, respectively, in the atherosclerotic group, and compared with the control group, the expression of TNF- 伪 was 83.836 卤9.442 卤9.442, respectively. Compared with the atherosclerotic group, the expression of IL-6 TNF- 伪 decreased in Ad-HGF group. Conclusion the increase of IL-6 TNF- 伪 expression in brain tissue of atherosclerotic rats may reduce the expression of IL-6TNF- 伪 in brain tissue of atherosclerotic rats. That is to inhibit atherosclerotic rat brain tissue inflammatory response.
【作者单位】：新乡医学院第一附属医院;
【基金】：河南省医学科技攻关省部共建项目(201401012)
【分类号】：R743

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