脑卒中血清游离DNA及脑源性神经营养因子水平变化及临床意义

发布时间：2018-03-23 04:25

本文选题：脑卒中　切入点：血清游离DNA　出处：《基因组学与应用生物学》2016年09期 　论文类型：期刊论文

【摘要】：为评估血清游离DNA及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)在脑卒中患者诊断中的临床价值,本研究收集131例脑卒中患者及50例健康体检者的外周血标本,提取血浆游离DNA,用SYBR Green染料实时荧光PCR方法检测血清游离DNA含量;采用酶联免疫吸附法检测血清BDNF含量,并检测血清同型半胱氨酸(Hcy)及超敏C反应蛋白(hs-CRP)。结果显示血清游离DNA、BDNF、Hcy、hs-CRP在脑梗死、脑出血患者及健康对照组三组间比较差异均有统计学意义(H分别为77.939,49.443,13.724,30.943;p0.001)。脑梗死患者游离DNA、Hcy、hs-CRP含量均高于健康对照组,差异均具有统计学意义(U分别为7.892,3.576,5.204;p0.05);脑出血患者DNA、Hcy、hs-CRP含量均高于健康对照组,差异均具有统计学意义(U分别为7.446,2.664,4.357;p0.001)。脑梗死及脑出血患者血清BDNF含量均低于健康对照组,差异具有统计学意义(U分别为-6.393,-5.794;p0.001)。脑梗死及脑出血患者两组间血清游离DNA、BDNF、Hcy、hs-CRP含量差异均无统计学意义(U分别为-1.483,0.936,0.119,-0.362;p0.05)。血清BDNF与脑卒中患者的年龄有关,其含量随患者年龄的增加而降低。血清游离DNA及BDNF在脑卒中患者的诊断中具有潜在的临床应用价值,其或可成为脑卒中诊断的有用指标。
[Abstract]:To evaluate the clinical value of serum free DNA and brain-derived neurotrophic factor (BDNF) in the diagnosis of stroke, the peripheral blood samples of 131 stroke patients and 50 healthy controls were collected. The free DNA was extracted from plasma and the content of free DNA in serum was detected by SYBR Green dye real-time fluorescence PCR, and the content of serum BDNF was detected by enzyme linked immunosorbent assay (Elisa). Serum homocysteine (Hcyn) and hypersensitive C-reactive protein (hs-CRP) were detected. The results showed that serum free DNA-BDNFN Hcyhs-CRP was found in cerebral infarction. The difference between the three groups was statistically significant (P < 0.05). The levels of Hcyhs-CRP in the patients with cerebral infarction were higher than those in the healthy control group (P < 0.05), and the levels of Hcyhs-CRP in the patients with cerebral infarction were significantly higher than those in the healthy control group (P < 0.05), and the levels of Hcyhs-CRP in the patients with cerebral infarction were significantly higher than those in the healthy controls. The levels of Hcyhs-CRP in patients with cerebral hemorrhage were significantly higher than those in healthy controls, and the differences were statistically significant. The serum BDNF levels in patients with cerebral infarction and intracerebral hemorrhage were lower than those in healthy controls, and the levels of Hcyhs-CRP in patients with cerebral hemorrhage were significantly higher than those in healthy controls, and the differences were statistically significant (7.446 卤2.6644.357p 0.001), respectively. The levels of serum BDNF in patients with cerebral infarction and intracerebral hemorrhage were lower than those in healthy controls. The difference was statistically significant between the two groups (-6.393) and 5.794% (p 0.001), respectively. There was no significant difference in serum BDNF between the two groups. Serum BDNF was related to the age of the patients with cerebral apoplexy, and there was no significant difference between the two groups (-1.4831,0.936 卤0.119- 0.362p0.05.), there was no significant difference in the content of serum BDNF between the two groups, and there was no significant difference in the levels of serum BDNF between the two groups, and there was no significant difference in the levels of serum BDNF between the two groups. Serum free DNA and BDNF have potential clinical application value in the diagnosis of stroke patients, which may be a useful index for the diagnosis of stroke.
【作者单位】：苏州大学附属第一医院检验科;
【分类号】：R743.3;R446.6;R440

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