视神经脊髓炎谱系疾病患者血清及脑脊液白介素-36α的表达及其临床意义

发布时间：2018-03-24 12:54

本文选题：视神经脊髓炎谱系疾病　切入点：白介素-36α　出处：《吉林大学》2017年硕士论文

【摘要】：目的:本研究旨在分析比较NMOSD患者与ONNDs组患者血清及脑脊液IL-36α表达及临床意义,并探讨其与NMOSD疾病严重程度的相关性,为揭示NMOSD的发病机制及寻求治疗靶点提供新的思路和方向。对象与方法:选取2015年1月至2016年8月之间就诊于吉林大学第一医院神经内科住院部的NMOSD患者73例,男性14例,女性59例。所有入组患者按最新NMOSD诊断标准经两位神经免疫专业医生诊断,均为临床确诊病例,并对入选患者进行扩展残疾状态量表(expanded disability status scale,EDSS)来判断神经功能缺损程度。同时选取性别、年龄匹配的ONNDs组患者50例。所有患者在样本采集前3个月内均未接受激素及免疫抑制剂治疗。所有患者均经本人及家属同意并签署知情同意书。1.双抗体夹心酶联免疫吸附方法(enzyme linked immunosorbent assay,ELISA)检测NMOSD患者血清及脑脊液中IL-36α的水平。2.分析血清及脑脊液中IL-36α的水平与NMOSD患者EDSS评分及年复发率(annualized relapse rate,ARR)的相关性。结果:1.NMOSD患者血清及脑脊液中IL-36α的水平较ONNDs组患者明显增高,差异有统计学意义(p=0.0335,p=0.0003)。2.NMOSD患者血清及脑脊液中IL-36α的水平与EDSS呈正相关(p=0.0412,p=0.0064);与ARR无明显相关性(p=0.4497,p=0.5473)。结论:NMOSD患者血清及脑脊液中IL-36α的水平较ONNDs组患者明显增高,提示促炎作用的细胞因子IL-36α可能在NMOSD的致病过程中发挥作用。NMOSD患者血清及脑脊液中IL-36α的水平与EDSS呈正相关,提示IL-36α可能成为评估NMOSD疾病严重程度的新的生物标志物。
[Abstract]:Objective: to analyze and compare the expression and clinical significance of IL-36 伪 in serum and cerebrospinal fluid (CSF) of patients with NMOSD and ONNDs, and to explore the relationship between IL-36 伪 expression and the severity of NMOSD. In order to reveal the pathogenesis of NMOSD and provide new ideas and directions for seeking therapeutic targets, 73 NMOSD patients, 14 males, were selected from January 2015 to August 2016 in Department of Neurology, first Hospital of Jilin University. 59 cases of female. All the patients in the group were diagnosed by two neuroimmunologists according to the latest diagnostic criteria of NMOSD, all of them were clinically confirmed cases. The selected patients were evaluated with expanded disability status scale EDSSs to determine the degree of neurological impairment. At the same time, gender was selected. All the patients were not treated with hormone and immunosuppressant within 3 months before sampling. All patients received informed consent form with the consent of themselves and their families. 1. Double antibody sandwich enzyme link. The level of IL-36 伪 in serum and cerebrospinal fluid (CSF) of NMOSD patients was detected by linked immunosorbent Elisa. 2. The correlation between IL-36 伪 level in serum and cerebrospinal fluid and EDSS score and annual recurrence rate of NMOSD patients was analyzed. The level of IL-36 伪 in liquid was significantly higher than that in ONNDs group. The level of IL-36 伪 in serum and cerebrospinal fluid was positively correlated with EDSS and had no significant correlation with ARR. Conclusion the level of IL-36 伪 in serum and cerebrospinal fluid of ONNDs patients was significantly higher than that in ONNDs group. The results suggest that IL-36 伪, the cytokine of proinflammatory effect, may play a role in the pathogenesis of NMOSD. The level of IL-36 伪 in serum and cerebrospinal fluid of NMOSD patients is positively correlated with EDSS, suggesting that IL-36 伪 may be a new biomarker for evaluating the severity of NMOSD disease.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R744.52

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