硫辛酸干预坐骨神经缺血再灌注损伤后的电生理变化

发布时间：2018-03-27 15:08

本文选题：硫辛酸　切入点：周围神经　出处：《中国组织工程研究》2017年12期

【摘要】：背景:硫辛酸具有由硫、碳原子所构成的封闭环状结构,具有强大的抗氧化能力,现已被广泛用于氧化应激、糖尿病性白内障、糖尿病多发性神经病及心血管损伤等糖尿病并发症等疾病的预防和治疗。目的:探究硫辛酸在周围神经缺血再灌注损伤中对周围神经功能的保护作用。方法:建立家兔周围神经缺血再灌注损伤模型,依据是否进行再灌注分为处理组与未处理组(不进行再灌注处理),处理组依据缺血时间1,3,6 h和再灌注前是否使用硫辛酸,于每个时间点又分别分为实验组与对照组,电镜观察各组实验动物坐骨神经超微结构的变化,并使用诱发电位仪检测坐骨神经电生理指标的变化。结果与结论:(1)随着缺血时间延长,轴突内空泡逐渐增多,轴突发生萎缩,轴突内微丝结构发生聚集并出现了明显的沃勒变性,而髓鞘则出现了增厚紊乱以及溶解现象。超微结构的破坏在缺血6 h时最为严重,沃勒变性及髓鞘溶解现象明显增加。对比实验组与对照组,硫辛酸的应用减少坐骨神经轴突中空泡数量,减轻了轴突的萎缩、沃勒变性以及脱髓鞘现象的产生;(2)随着缺血时间延长,各组实验动物坐骨神经的潜伏期显著增加,而波幅显著降低。潜伏期在缺血6 h达到最大值,而波幅在缺血6 h达到了最小值。对比未使用硫辛酸的对照组,实验组坐骨神经潜伏期明显下降而波幅明显增加;(3)结果说明,硫辛酸能够改善缺血再灌注造成的周围神经功能损伤。
[Abstract]:Background: lipoic acid has a closed ring structure composed of sulfur and carbon atoms and has a strong antioxidant capacity. It has been widely used in oxidative stress and diabetic cataract. Prevention and treatment of diabetic complications such as diabetic polyneuropathy and cardiovascular injury. Objective: to investigate the protective effect of lipoic acid on peripheral nerve function in peripheral nerve ischemia-reperfusion injury. A rabbit model of peripheral nerve ischemia-reperfusion injury was established. According to whether reperfusion was performed, the treatment group was divided into treatment group and untreated group (no reperfusion group). The treatment group was divided into experimental group and control group at each time point according to the time of ischemia and whether or not lipoic acid was used before reperfusion. The ultrastructure of sciatic nerve in each group was observed by electron microscope, and the changes of electrophysiological indexes of sciatic nerve were detected by evoked potentiometer. Results and conclusion: with the prolongation of ischemia time, the vacuoles in axons gradually increased and axon atrophy occurred. The microfilament structure in axon gathered and showed obvious Walller degeneration, while the myelin sheath showed thickening disorder and dissolving phenomenon. The damage of ultrastructure was the most serious at 6 h after ischemia. Compared with the control group, the application of lipoic acid reduced the number of axonal vacuoles of sciatic nerve and reduced the atrophy of axon. With the prolongation of ischemic time, the latency of sciatic nerve in each group increased significantly, but the amplitude decreased significantly, and the latency reached the maximum at 6 h after ischemia. Compared with the control group without lipoic acid, the latency of the sciatic nerve in the experimental group decreased significantly and the amplitude of the wave increased significantly. Lipoic acid can improve the injury of peripheral nerve function caused by ischemia reperfusion.
【作者单位】：沈阳医学院附属中心医院手外二科;中国医科大学肿瘤医院辽宁省肿瘤医院病理科;大连医科大学基础医学院病理教研室;沈阳医学院附属中心医院足踝外科;沈阳医学院;
【基金】：2012-2015年辽宁省科技厅课题(2012225019)~~
【分类号】：R745

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