Nurr-1对小胶质细胞微环境的影响

发布时间：2018-03-27 15:20

本文选题：小胶质细胞　切入点：Nurr　出处：《神经解剖学杂志》2017年02期

【摘要】：目的:探讨Nurr-1基因对小胶质细胞活化状态及其微环境的影响。方法:分离培养新生SD大鼠小胶质细胞,纯化、鉴定并分别用脂多糖(LPS)刺激活化和过表达Nurr1基因。实验随机分为小胶质细胞组、Nurr1过表达组、LPS处理组、Nurr1过表达+LPS处理组。ELISA分析过表达Nurr1基因对不同状态小胶质细胞分泌TNF-α、IL-1等炎症相关因子和BDNF、GDNF和PDNF等神经营养因子的影响。结果:(1)小胶质细胞混合培养、纯化、CD11b/c免疫细胞化学鉴定为阳性,纯度在95%;(2)Nurr1基因过表达小胶质细胞转染阳性率90%;(3)ELISA法检测结果显示:过表达Nurr1基因的小胶质细胞显著降低了TNF-α、IL-1的分泌,并促使了BDNF、GDNF和PDNF等神经营养因子的分泌。结论:Nurr1基因可抑制小胶质细胞的活化和减少炎症相关因子的产生,同时可促进GDNF、BDNF、PDNF等与DA能神经元存活和成熟等相关的神经营养因子的分泌。
[Abstract]:Objective: to investigate the effect of Nurr-1 gene on the activation state and microenvironment of microglia. Methods: microglia of newborn SD rats were isolated and purified. Lipopolysaccharide (LPS) was used to stimulate activation and overexpression of Nurr1 gene. The experiment was randomly divided into two groups: microglia group, Nurr1 overexpression group, LPS treatment group, and LPS treatment group. Elisa was used to analyze the expression of Nurr1 gene in different microglia. The effects of cytokines such as TNF- 伪 and IL-1 and neurotrophic factors such as BDNF-GDNF and PDNF on the secretion of TNF- 伪 IL-1 and neurotrophic factors were observed. Purified CD11b / c immunocytochemistry showed that the purity was 95%. The positive rate of transfection of Nurr1 gene into microglia cells was 90%. The results of Elisa showed that the overexpression of Nurr1 gene in microglial cells significantly decreased the secretion of TNF- 伪 and IL-1. Conclusion: 1. Nurr1 gene can inhibit the activation of microglia and reduce the production of inflammatory related factors. It can also promote the secretion of neurotrophic factors related to the survival and maturation of DA neurons, such as BDNFN PDNF.
【作者单位】：昆明医科大学第一附属医院神经外科;
【基金】：国家自然科学基金(81241126,81360197) 云南省教育厅科学研究基金项目(2013C227) 云南省科技厅-昆明医学院联合专项(2014FB041) 云南省干细胞与再生医学重点实验室开放课题(701160109)
【分类号】：R742.5

【相似文献】