细胞周期依赖激酶抑制剂Olomoucine对活化星形胶质细胞神经蛋白聚糖、短蛋白聚糖表达的抑制作用

发布时间：2018-03-28 06:34

本文选题：星形胶质细胞　切入点：Olomoucine　出处：《中风与神经疾病杂志》2017年06期

【摘要】：目的研究细胞周期依赖激酶抑制剂Olomoucine对星形胶质细胞活化后神经蛋白聚糖(Neurocan)、短蛋白聚糖(Brevican)表达的抑制作用。方法应用睫状神经营养因子(CNTF)刺激法建立体外培养星形胶质细胞的活化模型,实验分3组:对照组、活化组与抑制组。对照组:正常培养的星形胶质细胞;活化组:星形胶质细胞加入20 ng/ml睫状神经营养因子(CNTF)处理24 h;抑制组:星形胶质细胞用20 ng/ml睫状神经营养因子预处理24 h,加入100μmol/L Olomoucine。应用ELISA法检测不同时间点(12 h、24 h、48 h)各组细胞上清液中Neurocan、Brevican含量变化,半定量RT-PCR检测各组细胞GFAP mRNA及Neurocanm RNA、Brevican mRNA的表达变化。结果(1)活化组上清液中Neurocan、Brevican的含量在12 h、24 h、48 h随着时间的延长逐渐增加,与对照组比较差异有显著性(P0.01);抑制组上清液中Neurocan、Brevican含量随着时间的延长较活化组明显降低,与对照组、活化组比较差异有显著性(P0.01)。(2)活化组细胞GFAP mRNA、Neurocan mRNA、Brevican mRNA表达明显增加,与对照组比较差异有显著性(P0.01);抑制组细胞GFAP mRNA、Neurocan mRNA、Brevican mRNA表达均明显下调,与活化组比较差异有显著性(P0.01)。结论细胞周期依赖激酶抑制剂Olomoucine可抑制活化星形胶质细胞神经蛋白聚糖、短蛋白聚糖表达。
[Abstract]:Objective to study the inhibitory effect of cell cycle dependent kinase inhibitor (Olomoucine) on the expression of neuroproteoglycan neurocanine (Brevican) after astrocyte activation. Methods the cultured star was established by ciliary neurotrophic factor (ciliary neurotrophic factor) stimulation in vitro. The activation model of glial cells, The experiment was divided into three groups: control group, activated group and inhibitory group. Control group: normal cultured astrocytes; In activated group, astrocytes were treated with 20 ng/ml ciliary neurotrophic factor (CNTF) for 24 h, while in inhibitory group, astrocytes were pretreated with 20 ng/ml ciliary neurotrophic factor for 24 h and added 100 渭 mol/L Olomoucine. ELISA assay was used to detect 12 h and 24 h after treatment. The content of Neurocanium Brevican in the supernatant of cells in each group was changed for 48 h. Semi-quantitative RT-PCR was used to detect the expression of GFAP mRNA and Neurocanm RNA-Brevican mRNA. Results the content of Neurocann Brevican in supernatant of activated group increased gradually with the prolongation of time at 12 h, 24 h and 48 h, respectively. Compared with the control group, the content of Neurocann Brevican in the supernatant of the inhibitory group was significantly lower than that in the activated group, and the expression of GFAP mRNA-NeuromRNA-Brevican mRNA in the activated group was significantly higher than that in the control group. Compared with the control group, the expression of GFAP mRNA-neuromRNA-Brevican mRNA was significantly down-regulated in the inhibition group, and the difference was significant compared with the activated group. Conclusion Olomoucine, a cell cycle dependent kinase inhibitor, can inhibit the activation of astrocyte neuroproteoglycan. Short proteoglycan expression.
【作者单位】：南华大学附属第一医院神经内科;湖南省第二人民医院;南华大学机能实验室;
【基金】：衡阳市科技局(2015KJ43)
【分类号】：R741

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