2-氯化腺苷通过调节戊四氮点燃癫痫小鼠脑组织P糖蛋白水平减轻组织脑损伤

发布时间：2018-03-30 23:06

本文选题：-氯化腺苷(-CAdo)　切入点：戊四氮(PTZ)　出处：《细胞与分子免疫学杂志》2017年04期

【摘要】：目的观察2-氯化腺苷(2-CAdo)对戊四氮(PTZ)点燃癫痫小鼠脑内P糖蛋白(PGP)表达的影响。方法随机将C57BL/6小鼠40只,分为对照组(n=8)、PTZ组(n=16)和2-CAdo组(n=16),PTZ组和2-CAdo组小鼠腹腔注射30 mg/(kg·d)PTZ制备慢性点燃癫痫模型,对照组腹腔注射等量生理盐水,观察小鼠在PTZ点燃过程中癫痫发作情况(点燃率、点燃潜伏时间、发作开始时间和发作持续时间)。癫痫模型成功后,2-CAdo组小鼠连续腹腔注射2-CAdo[0.6 mg/(kg·d)]2周,对照组和PTZ组腹腔注射等量生理盐水。2周后将小鼠全部处死,采用HE染色观察小鼠大脑皮层和海马区的形态,Western blot法检测小鼠大脑皮层和海马中PGP水平,免疫组织化学染色检测PGP在小鼠大脑皮层和海马中的表达和定位。结果癫痫发作时,小鼠表现出全身颤抖、毛发竖起,精神萎靡、食欲不振、冲撞笼栏等异常行为。HE染色结果显示,2-CAdo组小鼠大脑皮层及海马脑组织损伤程度较PTZ组轻。在小鼠大脑皮层中,2-CAdo组PGP蛋白表达量明显低于对照组和PTZ组;而在海马中组织中,2-CAdo组和PTZ组PGP蛋白表达量又显著性高于对照组,尤其以2-CAdo组最为明显。结论 2-CAdo能够减轻癫痫所致的脑组织损伤,并且能下调大脑皮层PGP蛋白表达和上调海马区PGP蛋白的表达。
[Abstract]:Aim to observe the effect of 2-adenosine chloride 2-CAdol on the expression of P glycoprotein (P GP) in the brain of PTZ- kindled mice induced by pentylenetetrazol (PTZ).Methods Forty C57BL/6 mice were randomly divided into control group, 2-CAdo group and 2-CAdo group. The model of chronic kindled epilepsy was induced by intraperitoneal injection of 30 mg/(kg d)PTZ. The control group was given the same amount of normal saline intraperitoneally.The seizure rate, latent time, onset time and duration of PTZ kindling in mice were observed.The mice in the control group and the PTZ group were killed by intraperitoneal injection of 2-CAdo (0.6 mg/(kg d) for 2 weeks after the success of the epileptic model. The mice in the control group and the PTZ group were sacrificed after 2 weeks of intraperitoneal injection of the same amount of normal saline.The expression and localization of PGP in cerebral cortex and hippocampus of mice were detected by HE staining and immunohistochemical staining.Results during epileptic seizures, the mice showed shivering all over the body, hair erect, mental retardation, loss of appetite, collision cage bars and other abnormal behaviors. The results of HE staining showed that the damage degree of cerebral cortex and hippocampal brain tissue in group 2 CAdo was less than that in group PTZ.Conclusion 2-CAdo can attenuate the brain tissue injury induced by epilepsy and down-regulate the expression of PGP protein and up-regulate the expression of PGP protein in hippocampus.
【作者单位】：武汉市第一医院神经内科;武汉市第一医院急诊科;华中科技大学附属同济医院神经内科;
【基金】：湖北省卫计委课题(WJ2015MB134)
【分类号】：R742.1

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