低氧条件下PSB603干预对少突胶质前体细胞增殖和分化的影响

发布时间：2018-04-02 05:29

本文选题：少突胶质前体细胞　切入点：低氧　出处：《神经解剖学杂志》2017年02期

【摘要】：目的:探讨在低氧条件下,腺苷受体A2b(A2bAR)拮抗剂PSB603对少突胶质前体细胞(OPC)增殖和分化的影响。方法:剥离新生2 d SD大鼠的脑皮层,并原代培养其OPC,随机分为对照组、PSB603组、2%O_2组和实验组,实验组在2%O_2干预的基础上再给予PSB603处理,4 d后用BrdU掺入技术和免疫细胞化学方法检测BrdU阳性细胞百分数和CNPase阳性细胞百分数的变化,并用qRT-PCR技术检测各组细胞P21cip1、P27kip1 mRNA含量的变化。结果:与对照组相比较,2%O_2组的BrdU阳性细胞百分数显著上升(P0.01),P21、P27 mRNA含量均显著下调(P0.01),各组间CNPase阳性细胞百分数无明显变化;实验组与2%O_2组间各实验差异均无统计学意义(P0.05)。结论:低氧短时间干预能够促进OPC细胞的增殖,P21cip1和P27kip1参与其调控,A2b AR未改变低氧所诱导的OPC增殖。
[Abstract]:Aim: to investigate the effects of adenosine receptor A2bA2bAR-antagonist PSB603 on proliferation and differentiation of oligodendrocyte precursor cells (OPC) under hypoxia. OPC was randomly divided into two groups: the control group (PSB603) and the control group (PSB603). The experimental group was treated with PSB603 for 4 days on the basis of 2%O_2 intervention. The percentage of BrdU positive cells and the percentage of CNPase positive cells were detected by BrdU incorporation and immunocytochemistry. Results: compared with the control group, the percentage of BrdU positive cells in the control group increased significantly (P 0.01) and P21P 27 mRNA decreased significantly, but the percentage of CNPase positive cells did not change in each group. There was no significant difference between the experimental group and the 2%O_2 group (P 0.05). Conclusion: hypoxia for a short time can promote the proliferation of OPC cells; P21cip1 and P27kip1 participate in the regulation of OPC proliferation induced by hypoxia.
【作者单位】：宁夏医科大学宁夏颅脑疾病重点实验室;宁夏医科大学基础学院人体解剖与组织胚胎学系;宁夏医科大学公共卫生与管理学院流行病与卫生统计学系;宁夏医科大学总医院病理科;
【基金】：国家自然科学基金(31360240)
【分类号】：R741

【参考文献】