人脐带间充质干细胞不同移植途径治疗缺血性脑损伤以及生物发光技术示踪的研究及进展

发布时间：2018-04-03 22:28

本文选题：脐带　切入点：间质干细胞　出处：《中国组织工程研究》2017年33期

【摘要】：背景:人脐带间充质干细胞移植能有效促进缺血性脑损伤实验动物神经功能恢复,但目前尚不明确人脐带间充质干细胞的治疗机制及最佳移植途径。活体生物发光成像技术能实时动态监测移植干细胞在体内增殖、迁移及存活等情况,已广泛运用于干细胞移植领域。目的:综述人脐带间充质干细胞经不同移植途径治疗缺血性脑损伤的应用及活体生物发光成像技术在干细胞移植治疗缺血性脑损伤研究中的最新进展。方法:分别以"人脐带间充质干细胞,缺血性脑损伤,活体生物发光成像","human umbilical cord mesenchymal stem cells,brain ischemia,bioluminescence imaging"为检索词,由第一作者检索2011年1月至2016年12月相关期刊论文、Pub Med数据库相关文献。排除相关性差及重复性研究的文献,计算机初检到98篇文献,最终保留38篇进行归纳总结。结果与结论:人脐带间充质干细胞可经静脉、动脉、腰穿、脑立体定向移植、侧脑室移植等不同移植途径治疗缺血性脑损伤,各有优缺点,主要集中在干细胞迁移范围、存活率和安全性等方面。研究发现活体生物发光成像技术具有实时动态监测、高灵敏度、高时间分辨率、操作方便等优点,可用于监测移植干细胞在体内迁移、增殖和存活等情况。运用活体生物发光成像技术示踪人脐带间充质干细胞不同移植途径治疗缺血性脑损伤,明确人脐带间充质干细胞的作用机制及选择最佳移植途径值得进一步探讨。
[Abstract]:Background: transplantation of human umbilical cord mesenchymal stem cells can effectively promote the recovery of neural function in experimental animals with ischemic brain injury. However, the therapeutic mechanism and the best way of transplantation of human umbilical cord mesenchymal stem cells are unclear.In vivo bioluminescence imaging can dynamically monitor the proliferation, migration and survival of transplanted stem cells in vivo, and has been widely used in stem cell transplantation.Aim: to review the application of human umbilical cord mesenchymal stem cells (HMSCs) in the treatment of ischemic brain injury through different transplantation approaches and the recent progress in the study of stem cell transplantation in the treatment of ischemic brain injury.Methods: "Human umbilical cord mesenchymal stem cells, ischemic brain injury, in vivo bioluminescence imaging" and "human umbilical cord mesenchymal stem cells of brain ischemic cells imaging" were used as the key words, respectively.From January 2011 to December 2016, the Chinese journal full-text database Pub Med was searched by the first author.Excluding the literature of poor correlation and repeatability, 98 papers were first detected by computer, and 38 papers were retained to sum up.Results and conclusion: human umbilical cord mesenchymal stem cells can be transplanted through vein, artery, lumbar puncture, stereotactic brain transplantation and lateral ventricle transplantation to treat ischemic brain injury.Survival rate and safety.It is found that in vivo bioluminescence imaging has the advantages of real-time dynamic monitoring, high sensitivity, high time resolution and convenient operation. It can be used to monitor the migration, proliferation and survival of transplanted stem cells in vivo.In vivo bioluminescent imaging was used to trace different ways of transplantation of human umbilical cord mesenchymal stem cells in the treatment of ischemic brain injury. The mechanism of action of human umbilical cord mesenchymal stem cells and the selection of the best transplantation pathway are worthy of further discussion.
【作者单位】：广东省中医院;
【基金】：国家自然科学基金面上项目(81373569)~~
【分类号】：R741

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