大鼠脑缺血再灌注后海马组织中GFAP和p-STAT3表达研究

发布时间：2018-04-04 13:50

  本文选题：缺血再灌注　切入点：海马　出处：《军事医学》2016年11期

【摘要】：目的检测脑缺血再灌注(I/R)后海马齿状回区(dentate gyrus,DG)星形胶质细胞活化增殖及其磷酸化信号转导与转录激活子3(phosphorylated STAT3,p-STAT3)表达情况,为调控星形胶质细胞异常活化增殖提供理论依据。方法线栓法制作大鼠脑局部I/R模型,48只Sprague-Dawley(SD)大鼠随机分为假手术(Sham)组、I/R-24 h组、I/R-72 h组及I/R-7 d组。免疫荧光法及Western印迹检测缺血后不同时间点患侧海马区胶质原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)标记阳性细胞及p-STAT3表达情况。结果与Sham组比较,I/R-72 h和I/R-7 d组海马区GFAP蛋白表达增加(P0.05);随缺血时间延长,GFAP蛋白表达增加呈上升趋势,且24 h、72 h和7 d各时间点之间两两比较差异均具有统计学意义(P0.05)。与Sham组比较,脑I/R后海马区各时间点p-STAT3表达量均显著增加(P0.05);p-STAT3表达在24 h最高,72 h开始下降,7 d仍有一定量表达,与I/R-24 h组比较,I/R-72 h组和I/R-7 d组p-STAT3表达量均显著降低(P0.05)。结论脑I/R损伤后,海马区星形胶质细胞大量增殖及其p-STAT3表达增加;且p-STAT3的表达高峰在时间上早于星形胶质细胞的大量增殖。
[Abstract]:Objective to detect the activation and proliferation of astrocytes and the expression of phosphorylated signal transduction and activator 3(phosphorylated STAT3p-STAT3 in dentate gyrus of dentate gyrus after cerebral ischemia-reperfusion (I / R) so as to provide a theoretical basis for regulating the abnormal activation and proliferation of astrocytes.Methods 48 Sprague-Dawley SD rats were randomly divided into I / R 72 h group and I/R-7 d group.The expression of glial fibrillary acidic protein (p-STAT3) and glial fibrillary acidic protein (glial fibrillary acidic protein) positive cells were detected by immunofluorescence and Western blotting at different time points after ischemia.Results compared with Sham group, the expression of GFAP protein increased in hippocampus of I- / R-72 h group and I/R-7 d group, and the expression of GFAP protein increased with the prolongation of ischemia time, and there were significant differences between the two groups at 24 h and 7 d.涓嶴ham缁勬瘮杈，

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