内质网应激预处理对大鼠局灶性脑缺血再灌注后听皮层损伤的保护作用

发布时间：2018-04-05 21:43

本文选题：内质网应激　切入点：凋亡　出处：《实用医学杂志》2017年16期

【摘要】：目的探讨2-脱氧葡萄糖诱导内质网应激(ERS)预处理对大鼠局灶性脑缺血再灌注损伤后听力损伤的保护作用及机制。方法 SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(I/R组)、ERS预处理组。采用线栓法制备大鼠大脑中动脉栓塞模型,脑缺血60 min,再灌注24 h。行神经功能评分,测定脑梗死体积,检测听性脑干反应,HE染色法观察听皮层神经元病理变化,末端标记法观察神经细胞凋亡状况。免疫组化法测定GRP78、Caspase-12蛋白表达。结果与Sham组比较,I/R组神经功能缺失评分升高,脑梗死体积增加,ABR反应阈值明显增加,HE染色正常组织结构消失,凋亡指数显著增加,GRP78、Caspase-12蛋白表达明显上升。与I/R组比较,ERS预处理组各项指标均有不同程度的改善。结论 ERS预处理可以减轻缺血再灌注损伤及细胞凋亡,减轻听力损伤。
[Abstract]:Objective to investigate the protective effect and mechanism of 2-deoxyglucose induced endoplasmic reticulum stress (ERS) preconditioning on hearing loss after focal cerebral ischemia-reperfusion injury in rats.Methods Sprague-Dawley rats were randomly divided into sham group (sham group) and ischemia reperfusion group (I / R group).The middle cerebral artery (MCAE) embolization model of rats was established by the method of thread embolization. The rats were subjected to cerebral ischemia for 60 min and reperfusion for 24 h.Nerve function score, cerebral infarction volume, auditory brainstem reaction and HE staining were used to observe the pathological changes of auditory cortical neurons, and the apoptosis of neurons was observed by end labeling method.The expression of GRP78 Caspase-12 protein was detected by immunohistochemical method.Results compared with the Sham group, the neurological deficit score in the I / R group increased, the threshold of ABR response increased significantly, and the normal tissue structure disappeared, and the apoptotic index increased significantly the expression of Caspase-12 protein in the GRP78 was significantly increased.Compared with I / R group, the indexes of ERS preconditioning group were improved to some extent.Conclusion ERS preconditioning can attenuate ischemia reperfusion injury, cell apoptosis and hearing impairment.
【作者单位】：河北医科大学第二医院耳鼻咽喉科;河北医科大学第二医院骨科;
【基金】：河北省卫计委医学科学研究重点课题(编号:20160107)
【分类号】：R743.3;R764

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