运动干预对帕金森病大鼠黑质DJ-1的表达和抗氧化物酶的影响

发布时间：2018-04-06 02:22

本文选题：帕金森病　切入点：黑质　出处：《西北师范大学学报(自然科学版)》2017年03期

【摘要】：探讨早期运动干预对帕金森病(PD)模型大鼠黑质多巴胺(DA)能神经元的抗氧化保护机制.选清洁级SD大鼠64只分为4组:假手术组(Control,12只)、假手术运动组(Control+Ex,12只)、PD组(PD,20只)和PD运动组(PD+Ex,20只).单侧前脑内侧束(MFB)注射6-OHDA建立PD模型大鼠,运动组术后24h开始进行持续4周跑台训练,11m·min~(-1) ,30min·d~(-1) ,5d·(7d)~(-1) .采用阿扑吗啡(APO)诱导旋转检测行为变化,应用PCR和Western blotting检测黑质DJ-1变化,比色法检测黑质超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)指标.黑质DJ-1基因表达显示,PD+Ex组比PD组分别明显增加(P0.05);黑质DJ-1蛋白表达显示,PD+Ex组较PD组有明显提升(P0.05);PD组大鼠SOD、GSH-Px活力明显低于Control组(P0.01),MDA含量显著高于Control组(P0.01);PD+Ex组大鼠SOD、GSH-Px活力和MDA含量与Control组和PD组均有显著性差异(P0.05).表明早期运动干预能提高黑质DA能神经元DJ-1并增强抗氧化应激能力.
[Abstract]:To investigate the antioxidation mechanism of early exercise intervention on dopaminergic neurons in substantia nigra of PD model rats.Sixty-four SD rats of clean grade were divided into 4 groups: sham-operated group (n = 12), sham operation group (n = 12), control Excert group (n = 12), PD group (n = 20) and PD exercise group (n = 20).6-OHDA was injected into unilateral medial forebrain bundles to establish PD model rats. The rats in the exercise group were trained continuously for 4 weeks (11m / min / min) for 30 min / d ~ (-1)) for 5 days and 7 days / d respectively (P < 0.01). The rats in the exercise group were treated with treadmill training for 4 weeks at 24 hours after operation. The rats in the exercise group were treated with treadmill training for 4 weeks.PCR and Western blotting were used to detect the changes of DJ-1 in substantia nigra, and colorimetric method was used to detect the indexes of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in substantia nigra.There was significant difference in activity and MDA content between Control group and PD group (P 0.05).It was suggested that early exercise intervention could increase the DJ-1 of DA neurons in substantia nigra and enhance the ability of antioxidant stress.
【作者单位】：北京师范大学体育与运动学院;西北师范大学体育学院;
【基金】：国家自然科学基金资助项目(31340025,31571221)
【分类号】：R742.5

【相似文献】