牙髓干细胞移植治疗缺氧缺血性脑损伤新生大鼠远期行为学及环磷酸腺苷反应元件结合蛋白的变化

发布时间：2018-04-08 21:58

本文选题：牙髓　切入点：环AMP反应性元件结合蛋白　出处：《中国组织工程研究》2017年05期

【摘要】：背景:环磷酸腺苷反应元件结合蛋白(c AMP response element binding protein,CREB)是影响记忆存储的关键蛋白,与长期记忆密切相关,研究牙髓干细胞侧脑室移植远期行为学及CREB的变化机制可为缺氧缺血性脑损伤的治疗提供新思路。目的:观察人牙髓干细胞移植对缺氧缺血性脑损伤新生大鼠远期行为学以及CREB表达的影响,为缺氧缺血性脑损伤的治疗提供科学的理论依据。方法:36只健康7 d龄SD大鼠随机等分为正常组、缺氧缺血性脑损伤组和人牙髓干细胞组。采用经典的Rice法建立新生大鼠缺氧缺血性脑损伤模型,造模后24 h后,人牙髓干细胞组经侧脑室注入2μL人牙髓干细胞(浓度为1.5×106μL-1),正常组和缺氧缺血性脑损伤组分别注射等量生理盐水。结果与结论:人牙髓干细胞组大鼠平均觅水时间、平均逃避潜伏期及平均逃避距离亦显著短于缺氧缺血性脑损伤组(P0.01),但其平均觅水时间、逃避潜伏期及距离均长于正常组(P0.01);尼氏染色结果示人牙髓干细胞组海马CA1区细胞排列较规则,细胞数量较多,显著多于缺氧缺血性脑损伤组,但仍少于正常组(P0.05);免疫组织化学染色结果示人牙髓干细胞组CREB阳性细胞数亦显著多于缺氧缺血性脑损伤组,但仍显著少于正常组(P0.01)。提示人牙髓干细胞移植可通过促进海马CA1区CREB的表达,改善缺氧缺血性脑损伤新生大鼠远期学习记忆能力,从而修复新生大鼠缺氧缺血性脑损伤。
[Abstract]:Background: cyclic adenosine monophosphate response element binding protein (c AMP response element binding protein) is a key protein affecting memory storage and is closely related to long-term memory.To study the long term behavior and the mechanism of CREB in lateral ventricle transplantation of dental pulp stem cells may provide a new idea for the treatment of hypoxic ischemic brain injury.Aim: to observe the effects of human dental pulp stem cell transplantation on the long-term behavior and CREB expression in neonatal rats with hypoxic-ischemic brain injury (HIE) and to provide a scientific theoretical basis for the treatment of hypoxic-ischemic brain injury (HIE).Methods Thirty six healthy SD rats aged 7 days were randomly divided into normal group, hypoxic-ischemic brain injury group and human dental pulp stem cell group.The model of hypoxic-ischemic brain injury in neonatal rats was established by classical Rice method.2 渭 L human dental pulp stem cells (1.5 脳 106 渭 L -1) were injected into the lateral ventricle of human dental pulp stem cells group, and the same amount of normal saline was injected into normal group and hypoxic ischemic brain injury group.Results and conclusion: the average water seeking time, the average escape latency and the average escape distance in the human dental pulp stem cell group were significantly shorter than those in the hypoxic ischemic brain injury group (P 0.01), but the average water seeking time was also lower than that in the hypoxic ischemic brain injury group.The escape latency and distance were longer than that of the normal group (P 0.01), and the results of Nissl staining showed that the number of CA1 cells in the hippocampus of the human dental pulp stem cells group was more than that of the hypoxic-ischemic brain injury group, and the number of cells in the hippocampus was more than that in the hypoxic-ischemic brain injury group.The results of immunohistochemical staining showed that the number of CREB positive cells in human dental pulp stem cell group was significantly higher than that in hypoxic-ischemic brain injury group, but still significantly less than that in normal group.The results suggest that human dental pulp stem cell transplantation can improve the long-term learning and memory ability of neonatal rats with hypoxic-ischemic brain injury by promoting the expression of CREB in the CA1 area of hippocampus, thereby repairing the hypoxic-ischemic brain injury in newborn rats.
【作者单位】：潍坊医学院口腔医学院;潍坊医学院临床医学院血液科;潍坊医学院医学影像学系;
【基金】：国家自然科学基金项目(81000268) 山东省自然科学基金项目(ZR2014JL049);山东自然科学基金项目(ZR2014HQ077) 山东省医药卫生科技发展计划项目(2014WS0473)~~
【分类号】：R742

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