音乐对大鼠颞叶癫痫治疗作用的初步探究

发布时间：2018-04-09 17:14

本文选题：音乐　切入点：癫痫　出处：《华东师范大学》2014年硕士论文

【摘要】：癫痫是一种常见的中枢神经系统慢性疾病。虽然临床上已有多种治疗手段,但它们的有效性和实用性受到副作用、严重并发症、手术风险以及昂贵的费用等因素的限制。因此寻求更加安全、有效、并且经济上可承受的癫痫治疗/辅助治疗手段成为一项亟待的任务。本实验采用氯化锂-匹罗卡品癫痫大鼠模型来探究音乐对癫痫的治疗作用和及其神经机制。实验采用72只Wistar大鼠,随机分成4组：音乐组(n=21)、白噪音组(n=21)、癫痫对照组(n=21)和健康对照组(nn=9)。氯化锂-匹罗卡品注射过的大鼠一旦呈现癫痫持续发作状态后,即开始对音乐组和白噪音组分别播放莫扎特K488音乐和白噪音(75分贝,每天21点至次日9点连续播放12小时),共持续9周。采用24小时连续行为视频观察和EEG记录等手段,观察音乐环境对癫痫的影响。实验结果表明,音乐治疗6周后,音乐组的癫痫发作频率比癫痫组低78.33±7.01%(n=7),发作持续时间短33.65±4.45%(n=7),癫痫发作等级降低22.28±9.93%(n=7),发作间期棘波频率和幅值分别降低了25.18±6.81%(n=12)和12.58±3.71%(n=12)。此外,音乐对癫痫动物所表现出的认知功能障碍也有显著的改善作用。在新异物体识别实验中,癫痫组大鼠对于新异物体的偏好显著地(p0.05)低于健康组大鼠,其探索新异物体的时间比健康组短33.11±2.50%(n=6)：而音乐组动物的新异物体探索时间比癫痫组增长了28.57±7.18%(nn=7),达到了与健康组相同的水平(p0.05)。此外,音乐还可能具有缓解癫痫动物焦虑行为的作用。旷场实验中,癫痫组在中央区域活动时间显著高于健康组,而音乐组的这一指标呈现降低的趋势,虽然与癫痫组相比统计学上未达到显著性水平(p=0.059)。我们还发现,音乐可以提高抗癫痫药物拉莫三嗪的药效。我们的实验结果为音乐对癫痫及其所导致的认知功能障碍的治疗效果提供了充分的实验证据,对于治疗成人癫痫具有十分重要的临床意义。
[Abstract]:Epilepsy is a common chronic disease of the central nervous system. Although there are many kinds of clinical treatment, but effective and practical by their side effects, complications, operative risk and high cost and other factors. Therefore, seeking more secure, effective, and affordable treatment of epilepsy / adjuvant therapy means to become a key task. This experiment using lithium chloride pilocarpine induced epilepsy rat model to explore the effects of music therapy on epilepsy and its neural mechanism. The 72 Wistar rats were randomly divided into 4 groups: the music group (n=21), white noise group (n=21), epilepsy control group (n=21) and control group (nn=9). Lithium pilocarpine injected rats once showed seizures after the start of the music group and white noise group were playing Mozart K488 music and white noise (75 dB per. Day 21 to 9 the next day, 12 hours of continuous playback) lasted 9 weeks. The 24 hours of continuous observation and EEG behavior video recording and other means to observe the effect of environmental music on epilepsy. The experimental results show that the music after 6 weeks of treatment, the music group the seizure frequency of epilepsy group than low (n=7 78.33 + 7.01%), short duration of 33.65 + 4.45% attack (n=7), seizure reduced the levels of 22.28 + 9.93%, 25.18 + 6.81% (n=7) to reduce the interictal spike wave frequency and amplitude respectively (n=12) and 12.58 + 3.71% (n=12). In addition, the music show on the epilepsy animal cognitive dysfunction also improved significant effect. In the novel object recognition experiment, epilepsy rats for novel object preference (P0.05) was significantly lower than that of healthy rats, the time to explore the novel object than the healthy group 33.11 + 2.50% (n=6): new and different objects to explore the music group animal than epilepsy group Growth of 28.57 + 7.18% (nn=7), at the same level with the healthy group (P0.05). In addition, the music may also can alleviate the epilepsy animal anxiety behavior role. The open field test, the epilepsy group in the central area of activity time was significantly higher than that in healthy group, and the music group the index showed a decreasing trend although, compared with the epilepsy group did not reach statistical significant level (p=0.059). We also found that music can improve the efficacy of antiepileptic drug lamotrigine. Our experimental results provide experimental evidence for music on cognitive dysfunction caused by epilepsy and its treatment effect, it has very important clinical significance for treatment adult epilepsy.

【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742.1

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