垂体腺瘤中MUC1的表达与垂体腺瘤侵袭性的关系

发布时间：2018-04-10 11:45

本文选题：MUC1 + 垂体腺瘤　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的：探讨垂体腺瘤中MUC1的表达与垂体腺瘤侵袭性的关系。方法:采用免疫组化方法检测26例侵袭性垂体腺瘤和29例非侵袭性垂体腺瘤组织中MUC1的表达。将55例垂体腺瘤组织的标本全部经10%甲醛液固定后，进行石蜡包埋后，，再以5μm连续切片。做好切片常规给予脱蜡、水化后，用内源性过氧化物酶阻断剂和小牛血清分别在室温下孵育30分钟，以减低非特异性染色，排除干扰。检测标本均经微波法抗原修复。MUC1抗体（1:100）4摄氏度孵育过夜；生物素标记的二抗和抗生物素过氧化物酶连接物室温分别孵育30分钟；DAB显色1-3分钟；苏木素衬染，脱水、透明、封片、光镜观察。结果:侵袭性垂体腺瘤(IPA)组织中可见MUC1强阳性表达，阳性表达为深棕色、棕黄色颗粒，阳性信号主要位于细胞膜及胞浆内；非侵袭性垂体腺瘤(NIPA)可见弱阳性反应信号或未见MUC1表达。MUC1在侵袭性垂体腺瘤组织中的阳性表达率与非侵袭性垂体腺瘤组织之间存在显著性差异（分别76.9％和27.6%，P0.01）。结论: MUC1在侵袭性垂体腺瘤（IPA）组织中的阳性表达率与非侵袭性垂体腺瘤(NIPA)组织之间存在显著性差异，说明了MUC1在垂体腺瘤（PA）形成中可能有强烈的致瘤作用，并和肿瘤的侵袭性生长密切相关。因此检测MUC1在PA组织中的表达，有可能成为判断IPA的一种新的肿瘤标志物，对于临床医师诊判断垂体腺瘤侵袭性、治疗以及预后判断等都有会非常大的帮助。
[Abstract]:Objective : To investigate the relationship between expression of MUC1 in pituitary adenoma and invasion of pituitary adenoma .

Methods : Immunohistochemical method was used to detect the expression of MUC1 in 26 patients with invasive pituitary adenoma and 29 non - invasive pituitary adenoma .
the biotin - labeled di - and anti - biotin peroxidase linker were incubated at room temperature for 30 minutes , respectively ;
DAB is developed for 1 - 3 minutes ;
Subalpine lining dyeing , dehydration , transparency , sealing , light microscope observation .

Results : The positive expression of MUC1 was found in invasive pituitary adenoma ( IPA ) tissue , and the positive expression was dark brown and brown - yellow particles . The positive signal was mainly located in the cell membrane and cytoplasm .
Non - invasive pituitary adenoma ( NIPA ) showed weak positive response signal or no MUC1 expression . There was a significant difference between positive expression rate and non - invasive pituitary adenoma tissue in invasive pituitary adenoma ( 76.9 % vs 27 . 6 % , P0.01 ) .

Conclusion : The positive expression rate of MUC1 in invasive pituitary adenoma ( IPA ) tissue is significantly different from non - invasive pituitary adenoma ( NIPA ) .

【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

【参考文献】