白藜芦醇通过抑制CREB磷酸化减轻癫痫发作并改善认知功能

发布时间：2018-04-14 09:18

本文选题：颞叶癫痫 + 白藜芦醇　；参考：《实用医学杂志》2017年01期

【摘要】：目的:探讨白藜芦醇在颞叶癫痫(TLE)鼠中的抗癫痫和认知功能改善作用,及对颞叶皮质cAMP反应元件结合蛋白(CREB)磷酸化的影响。方法:雄性成年SD大鼠分为对照组、癫痫模型组和白藜芦醇干预组。氯化锂-匹罗卡品法建立TLE模型,干预组大鼠同时接受白藜芦醇灌胃给药,15 mg/(kg·d),连续7 d。模型点燃成功后观察动物行为,进行Racine评分。模型点燃成功及白藜芦醇给药第7天West-ern blot检测颞叶皮层CREB蛋白表达和磷酸化水平。Morris水迷宫检测学习记忆功能。结果:白藜芦醇干预显著降低了癫痫大鼠的Racine评分。癫痫大鼠颞叶皮层CREB蛋白表达水平虽无明显增加,但CREB磷酸化水平显著升高,白藜芦醇给药显著抑制了颞叶皮层的CREB磷酸化。癫痫大鼠第4、5、6天逃避潜伏期较对照组显著延长,目标象限停留时间则显著降低。与模型组比较,白藜芦醇干预组的逃避潜伏期显著降低,目标象限停留时间显著增加。结论:TLE大鼠中,白藜芦醇可有效抑制癫痫发作和改善认知功能,并可能通过抑制CREB磷酸化发挥其抗癫痫效应。
[Abstract]:Aim: to investigate the antiepileptic and cognitive effects of resveratrol in temporal lobe epilepsy rats and the effect of resveratrol on phosphorylation of cAMP response element binding protein (CREBB) in temporal lobe cortex.Methods: adult male SD rats were divided into control group, epileptic model group and resveratrol intervention group.TLE model was established by lithium-pilocarpine method. Rats in the intervention group received resveratrol intragastric administration for 15 mg/(kg d for 7 days.The animal behavior was observed and the Racine score was evaluated after successful kindling.West-ern blot was used to detect the expression of CREB protein and phosphorylation level of temporal cortex. Morris water maze was used to detect learning and memory function.Results: resveratrol significantly decreased the Racine score of epileptic rats.The expression of CREB protein in temporal cortex of epileptic rats was not significantly increased, but the level of CREB phosphorylation was significantly increased. Resveratrol administration significantly inhibited the phosphorylation of CREB in temporal cortex.The escape latency was significantly prolonged and the target quadrant residence time was significantly decreased in the rats with epilepsy on the 5th day of the 4th day compared with the control group.Compared with the model group, the escape latency of resveratrol intervention group decreased significantly, and the target quadrant residence time increased significantly.Conclusion resveratrol can effectively inhibit seizures and improve cognitive function in TLE rats. Resveratrol may play an antiepileptic effect by inhibiting CREB phosphorylation.
【作者单位】：重庆市江北区中医院神经外科;重庆市江北区中医院心血管内科;第三军医大学劳动卫生学教研室;
【基金】：国家自然科学基金青年科学基金项目(编号:31200627)
【分类号】：R742.1

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