LUC7L3与Ki-67在脑膜瘤组织中的表达及意义

发布时间：2018-04-15 11:09

本文选题：脑膜瘤 + LUC7L3　；参考：《南昌大学》2017年硕士论文

【摘要】：目的:对脑膜瘤组织中LUC7L3和Ki-67蛋白的表达情况进行检测和对比,对两种蛋白质在脑膜瘤的发生和发展中可能的作用机制进行探索,为脑膜瘤的诊断与治疗提供新的思路,从蛋白表达水平探索脑膜瘤的作用蛋白表达情况。方法:选择2007-2015年之间在南昌大学附属医院神经外科进行脑膜瘤切除手术的患者50例,另取20例正常脑膜组织作为对照,取自头外伤后行内外减压术的患者。将手术切除的组织进行病理学检测,并使用MaxVision TM免疫组化法检测LUC7L3蛋白以及Ki-67蛋白的表达情况。结合脑膜瘤病例的临床资料,对免疫组织化学结果进行分析、讨论。结果:(1)LUC7L3在不同等级的脑膜瘤组织中都有较高的阳性检出率(86%),但是在正常脑组织中LUC7L3的表达均为阴性,正常脑膜组织和脑膜瘤组织中LUC7l3的表达差异有显著性(?2=28.486,p0.05),并且研究结果显示LUC7L3的阳性率随肿瘤组织的恶性程度增加而升高,I级脑膜瘤组织的LUC7L3阳性率为71.4%,II级脑膜瘤组织的LUC7L3的阳性率为94%,III级脑膜瘤组织的LUC7L3的阳性率为100%,其阳性程度之间的差异有统计学意义(?2=21.45,p0.05)。(2)Ki-67在正常脑膜中表达阳性率低(LI1%),在脑膜瘤组织中表达阳性率显著升高,差异有统计学意义(?2=25.486,p0.05),Ki-67的与肿瘤组织的恶性程度也有正相关关系,本次研究结果显示,I级脑膜瘤组织的Ki-67标记指数平均为1%(0.00%~3.00%),II级脑膜瘤组织的Ki-67标记指数平均为3%(2.00%~4.25%),III级脑膜瘤组织的Ki-67的标记指数平均为6%(4.00%~20.00%),三组标记指数具有显著的统计学差异(?2=22.496,p0.01)(3)本次通过对数据进行Spearman相关性分析,分析结果显示LUC7L3与Ki-67值呈正相关关系(p0.05,r=0.845),并且结果显示LUC7L3与Ki-67的表达情况与脑膜瘤的病例分级有较大的相关性,与性别、年龄、肿瘤大小、发病部位无相关性(p均0.05)。结论:(1)LUC7L3和Ki-67在脑膜瘤组织中表达的阳性率及与肿瘤的恶性程度都有较高的相关关系,在正常的脑组织中很少发现LUC7L3和Ki-67的表达,说明LUC7L3和Ki-67的表达可能与脑膜瘤的发生和发展有密切联系;(2)LUC7L3和Ki-67在脑膜瘤组织中的阳性表达率都较高,并且随着脑膜瘤恶性程度的升高而升高,与脑膜瘤的恶性程度具有正相关关系,检测这两类蛋白量可以在脑膜瘤临床病理学分级中作为重要的参考指标;(3)LUC7L3当前研究较少,但是本次研究证明了LUC7L3与脑膜瘤发生和发展有正相关关系,所以可以通过抑制LUC7L3的表达抑制脑膜瘤的发生和发展。进一步研究LUC7L3与Ki-67在脑膜瘤发生、发展中的作用机制,可能为脑膜瘤的治疗提供新的途径。
[Abstract]:Objective: to detect and compare the expression of LUC7L3 and Ki-67 proteins in meningiomas, and to explore the possible mechanism of the two proteins in the occurrence and development of meningiomas, so as to provide a new idea for the diagnosis and treatment of meningiomas.To explore the expression of functional protein in meningioma from the level of protein expression.Methods: from 2007 to 2015, 50 patients underwent meningioma resection in the neurosurgery department of Nanchang University affiliated Hospital, and another 20 normal meningeal tissues were taken from the patients undergoing internal and external decompression after head trauma.The expression of LUC7L3 protein and Ki-67 protein were detected by MaxVision TM immunohistochemical method.Combined with the clinical data of meningioma cases, the immunohistochemical results were analyzed and discussed.Results the positive rate of LUC7L3 was higher in meningiomas of different grades, but the expression of LUC7L3 was negative in normal brain tissues.There was significant difference in the expression of LUC7l3 between normal meningeal tissue and meningioma tissue. The results showed that the positive rate of LUC7L3 increased with the increase of malignant degree of tumor tissue. The positive rate of LUC7L3 in grade I meningioma was 71.4%.The positive rate of LUC7L3 was 940.The positive rate of LUC7L3 in the meningioma of grade III was 100. The difference of the positive degree was statistically significant. The positive rate of LUC7L3 in normal meninges was lower than that in normal meninges. The positive rate of LUC7L3 in meningiomas was significantly higher than that in meningiomas.There was a positive correlation between Ki-67 and malignant degree of tumor tissue.The results of this study showed that the average Ki-67 labeling index of grade I meningioma tissue was 1: 0. 00 and the Ki-67 labeling index of grade II meningioma tissue was 3 ~ 2. 00 on average, the Ki-67 labeling index of meningioma tissue of grade I was 6 ~ 4. 00 on average, and the labeling index of Ki-67 in the three groups was significant.This time, the Spearman correlation analysis of the data was carried out.The results showed that there was a positive correlation between LUC7L3 and Ki-67, and the expression of LUC7L3 and Ki-67 was significantly correlated with the grade of meningioma, but not with sex, age, tumor size and location of meningioma.Conclusion the positive expression rate of LUC7L3 and Ki-67 in meningioma tissues and the degree of malignancy of meningiomas are highly correlated, and the expression of LUC7L3 and Ki-67 in normal brain tissues is rarely found.It is suggested that the expression of LUC7L3 and Ki-67 may be closely related to the occurrence and development of meningioma. The positive rates of LUC7L3 and Ki-67 in meningioma tissues are higher than those in meningioma tissues, and increase with the increase of malignant degree of meningioma.There is a positive correlation between the malignant degree of meningioma and the detection of these two kinds of proteins, which can be used as an important reference index in the clinicopathological grading of meningioma.But this study proves that LUC7L3 has a positive correlation with the occurrence and development of meningioma, so it can inhibit the occurrence and development of meningioma by inhibiting the expression of LUC7L3.Further study on the role of LUC7L3 and Ki-67 in the pathogenesis and development of meningioma may provide a new approach for the treatment of meningioma.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.45

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