不同缺血再灌注时间对脑梗死大鼠运动及认知功能的影响

发布时间：2018-04-16 22:01

本文选题：短暂性大脑中动脉闭塞 + 缺血再灌注　；参考：《中国康复医学杂志》2017年05期

【摘要】：目的:比较不同缺血再灌注时间对短暂性大脑中动脉闭塞(transient middle cerebral artery occlusion,tMCAO)大鼠的运动及认知功能的影响。方法:取穿梭箱主动回避率超过70%的大鼠60只随机分为4组:根据Bederson评分及存活率缺血再灌注60min组(n=8)、90min组(n=11)、120min组(n=7)及假手术组(n=7)纳入最终行为学研究。术后28天行TTC染色计算梗死体积。于术后第1、3、7、14、21、28天进行改良神经功能缺损程度(mNSS)评分,第3、7、14、21、28天进行前肢抓握力测试;于术后第14、21、28天进行穿梭箱测试。结果:缺血再灌注时间越长,大鼠的生存率越低(χ~2=12.694,P=0.005);梗死体积随缺血再灌注时间增加而增大(F=14.056,P=0.01)。缺血时间越长,mNSS评分越高(F=9.100,P=0.001);梗死组较假手术组抓握力量减小(F=11.630,P0.05);穿梭箱实验显示各时间点90min组主动回避率较假手术组降低(P0.05),120min组第14、21天时较假手术组主动回避率降低(P0.05)。结论:tMCAO大鼠随缺血再灌注时间延长,运动功能障碍严重程度增加,缺血60min再灌注不出现认知功能障碍,且因运动功能自发性恢复速度快,不适用于认知功能及长期运动功能行为学测试。缺血90min、120min出现认知功能障碍。
[Abstract]:Aim: to compare the effects of different ischemia-reperfusion time on motor and cognitive function in transient middle cerebral artery occlusion MCAO rats.Methods: sixty rats with active avoidance rate more than 70% were randomly divided into 4 groups: according to the Bederson score and survival rate of ischemia reperfusion 60min group (n = 80) and sham operation group (n = 70) were included in the final behavioral study.Infarct volume was calculated by TTC staining 28 days after operation.The mNSS score was performed on the 1st day, the 3rd day, 1421 and 28 days after operation, and the shuttle box test was carried out on the 141th day after the operation, and on the 28th day after operation, the score of the modified neural function defect was evaluated by mNSS, and the forelimb grip strength was measured on the 28th day after the operation.Results: the longer the ischemia-reperfusion time was, the lower the survival rate of rats was (蠂 ~ (2 +) 12.694) P ~ (0.005), and the infarct volume increased with the increase of ischemia-reperfusion time.The longer the ischemic time was, the higher the score of F9. 100 and P0. 001 was, the less the grip strength of the infarction group was than that of the sham-operated group, the lower the active avoidance rate of the 90min group at each time point was compared with that of the sham operation group at the 1421 day, and the lower the active avoidance rate of the sham operation group was (P 0. 05) than that of the sham-operated group at 1421 days after the operation, the shuttle box experiment showed that the active avoidance rate of the 90min group was lower than that of the sham-operated group at 1421 days.Conclusion with the prolongation of ischemia-reperfusion time, the severity of motor dysfunction increases in the rats with 60min ischemia reperfusion, and the cognitive dysfunction is not found in ischemia 60min reperfusion, and the spontaneous recovery rate of motor function is faster than that of MCAO rats.Not suitable for cognitive function and long-term motor function behavior test.Cognitive impairment occurred at 90 min and 120 min after ischemia.
【作者单位】：中山大学附属第三医院康复科;
【基金】：国家自然科学基金面上项目(81472156;81672259)
【分类号】：R743.3

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