有丝分裂原活化蛋白激酶信号通路介导大鼠星形胶质细胞氧糖剥夺后水通道蛋白4的表达

发布时间：2018-04-16 22:02

本文选题：氧糖剥夺 + 星形胶质细胞　；参考：《中国康复理论与实践》2017年12期

【摘要】：目的探讨有丝分裂原活化蛋白激酶(MAPKs)是否介导缺血后大鼠星形胶质细胞水通道蛋白4(AQP4)的表达。方法分离培养新生Sprague-Dawley大鼠脑星形胶质细胞。第2代细胞分成对照组、氧糖剥夺(OGD)组和阻断组。后两组复制OGD 5 h后复氧模型,12 h后阻断组分别换入含U0126(1μmol/L和10μmol/L,U1组和U10组)、SP600125(1μmol/L和10μmol/L,SP1组和SP10组)和SB203580(1μmol/L和10μmol/L,SB1组和SB10组)的培养液。复氧后0.5 h、1 h、1.5 h、2 h、3 h、4 h、8 h和12 h检测OGD组细胞体积,复氧后0.5 h、1 h、1.5 h、8 h和12 h Western blotting法检测OGD组AQP4表达;复氧后24 h,检测各组乳酸脱氢酶(LDH)活性,Western blotting法检测各组AQP4,磷酸化细胞外调节蛋白激酶(p-ERK)、c-Jun氨基末端激酶(p-JNK)和p38MAPK(p-p38 MAPK)表达。结果复氧后1.5 h、2 h、3 h和4 h时,OGD组细胞体积较对照组显著增大(P0.001),OGD组AQP4水平较对照组显著升高(P0.001),并在复氧后1.5 h达到峰值。OGD组p-ERK、p-JNK和p-p38 MAPK、AQP4水平较对照组显著增加(P0.001),阻断组p-ERK、p-JNK和p-p38 MAPK较OGD组显著下降(P0.001),SB10组AQP4较OGD组显著下降(P0.001)。除SP1组、SB1组外,各干预组LDH活性较OGD组显著下降(P0.01),SB10组最低(P0.001)。结论 MAPK信号通路,特别是p38MAPK可介导大鼠星形胶质细胞AQP4蛋白表达,加重细胞坏死。
[Abstract]:Aim to investigate whether mitogen activated protein kinase (MAPKs) mediates the expression of aquaporin 4 (AQP4) in rat astrocytes after ischemia.Methods the astrocytes of newborn Sprague-Dawley rats were isolated and cultured.The second passage cells were divided into control group, OGD group and blocking group.After 5 h of OGD replication and 12 h after reoxygenation, the blocking group was replaced with the culture medium containing U0126 1 渭 mol/L and 10 渭 mol / L LU1 and U10 group (SP600125 渭 mol/L and 10 渭 mol / L LSP1 and SP10 group), SB203580(1 渭 mol/L, 10 渭 mol / L LSB1 group and SB10 group, respectively.The cell volume of OGD group was measured at 0.5 h, 1 h, 1 h, 1 h, 1 h, 2 h and 3 h, and 4 h and 12 h after reoxygenation, and the expression of AQP4 in OGD group was detected by Western blotting assay at 1 h, 1 h, 1 h, 1 h and 12 h after reoxygenation.At 24 h after reoxygenation, the activity of lactate dehydrogenase (LDH) and the expression of extracellular regulated protein kinase (PERK), c-Jun amino-terminal kinase (c-Jun) and p38MAPK(p-p38 (MAPK) were detected by Western blotting assay.Results the cell volume in OGD group was significantly higher than that in control group at 1.5 h, 2 h, 3 h and 4 h after reoxygenation, and the level of AQP4 in OGD group was significantly higher than that in control group, and the levels of p-ERKN p-JNK and p-p38 MAPKN AQP4 in OGD group were significantly higher than those in control group at 1.5 h after reoxygenation.Compared with OGD group, p-ERKN p-JNK and p-p38 MAPK decreased significantly (P 0.001) in SB10 group and OGD group (P 0.001).The activity of LDH in the intervention group was significantly lower than that in the OGD group except the SP1 group (P 0.01) and SB10 group (P 0.001).Conclusion MAPK signaling pathway, especially p38MAPK, can mediate the expression of AQP4 protein in astrocytes and aggravate cell necrosis.
【作者单位】：陕西省核工业215医院神经外科;
【分类号】：R741

【相似文献】