胶质瘤中SPINT2基因的表达及其甲基化状态研究

发布时间：2018-04-17 01:11

本文选题：胶质瘤(gliomas) + 肝细胞生长因子激活物抑制剂(SPINT2)　；参考：《中南大学》2014年硕士论文

【摘要】：目的：检测正常脑组织标本及不同WHO分级的人脑胶质瘤标本中SPINT2基因的表达及其甲基化状态,探讨SPINT2基因甲基化对胶质瘤发生、发展过程的影响。方法：选取8例正常脑组织及24例不同病理等级的胶质瘤组织,用western blot方法检测各标本中SPINT2基因表达情况；同时使用甲基化特异性PCR(MSP)检测SPINT2基因在正常脑组织和胶质瘤中启动子甲基化情况。结果：1.Western-blot检测正常脑组织、胶质瘤组织中SPINT2蛋白表达实验结果：正常脑组织中SPINT2蛋白与低级别胶质瘤差异具有统计学意义(P=0.008)；正常脑组织中SPINT2蛋白与高级别胶质瘤差异具有统计学意义(P=0.000)；低级别胶质瘤中SPINT2蛋白与高级别胶质瘤差异无统计学意义(P=0.061)。2. MSP检测正常脑组织、胶质瘤组织中SPINT2基因甲基化状态实验结果：正常脑组织中SPINT2基因启动子甲基化率与低级别胶质瘤差异无统计学意义(P=0.347)；正常脑组织中SPINT2基因启动子甲基化率与高级别胶质瘤差异具有统计学意义(P=0.024)；低级别胶质瘤中SPINT2基因启动子甲基化率与高级别胶质瘤差异无统计学意义(P=0.097)。结论：1.SPINT2基因在所有正常脑组织及大部分胶质瘤组织中均可表达；正常脑组织中SPINT2蛋白含量明显高于胶质瘤组织；低级别胶质瘤组织中SPINT2蛋白含量与高级别胶质瘤组织相比无明显差异。2.正常脑组织中未发现SPINT2基因启动子出现甲基化现象；正常脑组织中SPINT2基因启动子甲基化率明显低于高级别胶质瘤组织；而正常脑组织中SPINT2基因启动子甲基化率与低级别胶质瘤组织、低级别胶质瘤组织中SPINT2基因启动子甲基化率与高级别胶质瘤组织相比无明显差异。图7幅,表6个,参考文献32篇。
[Abstract]:Aim: to detect the expression and methylation status of SPINT2 gene in normal brain tissues and human gliomas with different WHO grades, and to investigate the effect of methylation of SPINT2 gene on the occurrence and development of gliomas.Methods: the expression of SPINT2 gene in 8 normal brain tissues and 24 gliomas with different pathological grades was detected by western blot method.The promoter methylation of SPINT2 gene in normal brain tissue and glioma was detected by methylation specific PCR MSPs.Results 1. Western-blot was used to detect normal brain tissue.The expression of SPINT2 protein in normal brain tissue was significantly different from that in low-grade glioma (P < 0.008), SPINT2 protein in normal brain tissue was significantly higher than that in high-grade glioma (P = 0.000), and low grade glioma was lower grade (P < 0.05).There was no significant difference between SPINT2 protein and high grade glioma.MSP was used to detect normal brain tissue.Results of methylation status test of SPINT2 gene in glioma tissues: the methylation rate of SPINT2 gene promoter in normal brain tissue was not significantly different from that in low-grade glioma tissue, and the methylation rate of SPINT2 gene promoter in normal brain tissue was higher than that in low-grade glioma tissue.The methylation rate of SPINT2 gene promoter in low grade gliomas was not significantly different from that in high grade gliomas.Conclusion: 1. SPINT2 gene can be expressed in all normal brain tissues and most glioma tissues, and the content of SPINT2 protein in normal brain tissue is significantly higher than that in glioma tissue.There was no significant difference in SPINT2 protein content between low grade gliomas and high grade gliomas.No methylation of SPINT2 gene promoter was found in normal brain tissue, and the methylation rate of SPINT2 gene promoter in normal brain tissue was significantly lower than that in high grade glioma tissue.However, the methylation rate of SPINT2 gene promoter in normal brain tissue was not significantly different from that in low-grade glioma tissue and low-grade glioma tissue, and the methylation rate of SPINT2 gene promoter in low-grade glioma tissue was not significantly different from that in high-grade glioma tissue.There are 7 figures, 6 tables and 32 references.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

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