脊髓CXCR2在CCI大鼠神经病理性疼痛中的作用研究

发布时间：2018-04-17 10:12

  本文选题：神经病理性疼痛 + 趋化因子受体　； 参考：《中国现代医学杂志》2017年14期

【摘要】：目的 探讨脊髓趋化因子受体2(CXCR2)在坐骨神经慢性压迫(CCI)模型大鼠神经病理性疼痛(NPP)中的作用。方法 18只成年Sprague-Dawley雄性大鼠,随机分为3组,每组6只。对照组:大鼠不做任何处理;模型组:复制CCI大鼠模型;实验组:复制CCI大鼠模型后第3天腹腔注射漆树酸5 mg/kg。于复制大鼠CCI模型前1天,以及第1、3、5和7天分别测定大鼠后足热痛阈及机械痛阈,并应用Western blot测定腰段脊髓CXCR2的蛋白表达。结果 CCI大鼠术后第3天开始术侧下肢热痛阈值降低,模型组、实验组术侧下肢热痛阈值与对照组比较,差异有统计学意义(P0.05);实验组大鼠第5和7天术侧热痛阈值与模型组比较,差异有统计学意义(P0.05)。机械痛阈变化趋势与热痛阈一致。模型组、实验组大鼠脊髓CXCR2表达与对照组比较,差异有统计学意义(P0.05);实验组CXCR2表达与模型组比较,差异有统计学意义(P0.05)。结论 脊髓CXCR2表达的异常上调,可导致NPP产生和维持。腹腔注射漆树酸能够部分抑制CCI大鼠脊髓腰段CXCR2的表达上调,并缓解CCI大鼠的疼痛行为。
[Abstract]:Objective to investigate the role of CXCR2 in neuropathic pain induced by chronic compression of sciatic nerve in rats.Methods 18 adult Sprague-Dawley male rats were randomly divided into 3 groups with 6 rats in each group.Control group: rats did not do any treatment; model group: CCI rat model; experimental group: 3 days after CCI rat model was intraperitoneally injected with 5 mg 路kg ~ (-1) of lactoic acid.The heat pain threshold and mechanical pain threshold of the hind foot were measured one day before the CCI model was established, and the expression of CXCR2 protein in the lumbar spinal cord was measured by Western blot.Results the lower extremity thermal pain threshold of the CCI rats was decreased from the 3rd day after operation. The thermal pain threshold of the model group and the experimental group was compared with that of the control group.There was significant difference between the experimental group and the model group on the 5th and 7th day, the difference was statistically significant (P 0.05).The change trend of mechanical pain threshold is consistent with thermal pain threshold.In the model group, the expression of CXCR2 in the spinal cord of the experimental group was significantly higher than that in the control group (P 0.05), while the expression of CXCR2 in the experimental group was significantly higher than that in the model group (P 0.05).Conclusion the abnormal upregulation of CXCR2 expression in spinal cord may lead to the production and maintenance of NPP.Intraperitoneal injection of lactoxylic acid partially inhibited the up-regulation of CXCR2 expression in spinal cord of CCI rats and alleviated the pain behavior of CCI rats.
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