强化运动训练对卒中后抑郁大鼠抑郁程度及海马区kalirin-7表达的影响

发布时间：2018-04-17 19:20

本文选题：强化训练 + 脑卒中　；参考：《青岛大学》2016年硕士论文

【摘要】：目的观察强化训练对脑卒中后抑郁大鼠抑郁程度及海马区kalirin-7表达的影响,探讨强化训练改善卒中后抑郁大鼠抑郁程度可能的机制。方法取40只雄性Wistar大鼠,采用随机数字法将大鼠分为假手术组、脑卒中后抑郁(PSD)组、普通训练组、强化训练组,每组10只。PSD组、普通训练组、强化训练组采用线栓法建立左侧大脑中动脉阻塞(MCAO)模型,并给予慢性不可预知性应激刺激(CMS),假手术组仅分离颈总、颈内、颈外动脉,不做插线处理,也不给予CMS。造模成功后,假手术组与PSD组不进行跑台训练,普通训练组、强化训练组分别进行普通和强化跑台训练。4组大鼠分别于训练前和训练14d、28d后进行行为学实验【糖水消耗实验(SPT)、旷场实验(OFT)、强迫游泳实验(FST)】,然后采用western blot法检测其左侧海马区kalirin-7蛋白的表达情况。结果训练14d后,PSD组、普通训练组、强化训练组各项行为学实验结果与假手术组相比,差异均有统计学意义(P0.05),普通训练组的OFT和FST结果分别为(55.71±3.94)s和(67.42±3.64)s,与PSD组相比,差异均有统计学意义(P0.05),强化训练组各项行为学实验结果与PSD组和普通训练组相比,差异均有统计学意义(P0.05);训练28d后,PSD组、普通训练组各项行为学实验结果与假手术组相比,差异均有统计学意义(P0.05),普通训练组和强化训练组各项行为学实验结果与PSD组相比,差异均有统计学意义(P0.05),且强化训练组各项行为学实验结果与普通训练组相比,差异均有统计学意义(P0.05)。Western Blot结果显示,普通训练组、强化训练组海马区kalirin-7的表达均显著高于PSD组同时间点(P0.05);强化训练组海马区kalirin-7表达显著高于普通训练组同时间点(P0.05)。结论强化训练可改善卒中后抑郁大鼠的抑郁程度,增加海马区kalirin-7的表达,促进其神经功能的恢复,并且强化训练的效果优于普通训练。
[Abstract]:Objective to observe the effect of intensive training on depression degree and kalirin-7 expression in hippocampus of rats with post-stroke depression, and to explore the possible mechanism of intensive training in improving depression degree of post-stroke depression rats.Methods Forty male Wistar rats were randomly divided into sham-operated group, post-stroke depression group, general training group, intensive training group, 10 rats in each group, and general training group.In the intensive training group, the left middle cerebral artery occlusion (MCAO) model was established by the method of thread embolization, and chronic unpredictable stress stimulation was given to CMSO. In the sham operation group, the total cervical artery, internal carotid artery and external carotid artery were only separated, and no wire insertion or CMS was given to the sham operation group.After successful modeling, the sham-operation group and PSD group did not run platform training, the general training group,The rats in the intensive training group were treated with normal and intensive treadmill training respectively. The behavioral experiments were performed before training and 14 days after training respectively [sugar water consumption test (SPT), open field experiment (OFTT), forced swimming experiment (FST)].Then western blot method was used to detect the expression of kalirin-7 protein in the left hippocampus.Results after 14 days of training, the results of behavioral experiments in PSD group, general training group and intensive training group were significantly different from those in sham-operation group. The results of OFT and FST in general training group were 55.71 卤3.94s and 67.42 卤3.64s, respectively, compared with PSD group.The results of behavioral experiments in the intensive training group were significantly higher than those in the PSD group and the general training group, and were compared with those in the sham operation group after 28 days of training.The results of behavioral experiments in general training group and intensive training group were significantly different from those in PSD group, and the behavioral experiment results in intensive training group were higher than those in general training group.The results showed that the expression of kalirin-7 in hippocampus of general training group and intensive training group was significantly higher than that of PSD group at the same time point, and the expression of kalirin-7 in hippocampus of intensive training group was significantly higher than that of general training group at the same time point.Conclusion intensive training can improve the depression degree of post-stroke depression rats, increase the expression of kalirin-7 in hippocampus and promote the recovery of neural function, and the effect of intensive training is better than that of general training.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R743.3;R749.4

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