内源性大麻素含量升高可激活脊髓背角星形胶质细胞并导致机械性触诱发痛

发布时间：2018-04-18 19:30

本文选题：-AG + 内源性大麻素系统　；参考：《神经解剖学杂志》2017年01期

【摘要】：目的:观察丁高大鼠鞘内大麻素水平后实验动物的疼痛行为学变化及脊髓背角星形胶质细胞的激活状态,探讨内源性大麻素参与触诱发痛的可能机制。方法:成功建立大鼠鞘内置管模型后,分别鞘内注射外源性大麻素2-AG、大麻素受体激动剂CP55940、大麻素水解酶抑制剂JZL195。使用Von-frey纤维丝观察给药后不同时间点大鼠机械性缩足反射阈值的变化,运用共聚焦显影观察脊髓背角星形胶质细胞的激活状态。结果:(1)鞘内注射2-AG、CP55940、JZLl95后1 d即产生明显的触诱发痛(P0.01)并至少持续到给药后第21 d(P0.001);(2)鞘内注射2-AG、CP55940和JZLl95后第5 d即有明显的星形胶质细胞激活(P0.01),并可持续到给药后第21 d(P0.01)。结论:升高鞘内大麻素水平可能通过激活星形胶质细胞导致触诱发痛。
[Abstract]:Aim: to observe the changes of pain behavior and the activation of astrocytes in the dorsal horn of spinal cord after intrathecal cannabinoid level in tall rats, and to explore the possible mechanism of endogenous cannabinoids involved in tactile pain.Methods: the rat model of intrathecal tube placement was successfully established. Exogenous cannabinoid 2-AGN, cannabinoid receptor agonist CP55940 and cannabinoid hydrolase inhibitor JZL195 were injected intrathecally respectively.Von-frey filaments were used to observe the changes of mechanical reflex threshold in rats at different time points after administration and confocal development was used to observe the activation of astrocytes in the dorsal horn of spinal cord.Results one day after intrathecal injection of 2-AGN CP55940 (JZLl95), obvious touch-induced pain occurred (P0.01), and at least until the 21st day after administration, there were obvious astrocytic activation P0.01C on the 5th day after intrathecal injection of 2-AGN CP55940 and JZLl95, and it could be sustained until the 21st day after administration.Conclusion: the increase of intrathecal cannabinoid level may lead to tactile pain by activating astrocytes.
【作者单位】：第四军医大学西京医院疼痛科;
【基金】：国家自然科学基金(31530090,81471139)
【分类号】：R741

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