Hcy代谢关键酶MTHFR、CBS基因与缺血性脑卒中相关性研究的Meta分析

发布时间：2018-04-24 01:30

本文选题：MTHFR + C677T基因　；参考：《吉林大学》2014年硕士论文

【摘要】：目的：采用Meta分析的方法对Hcy代谢关键酶MTHFR、CBS基因与缺血性脑卒中的相关文献进行综合分析，判定MTHFR、CBS基因突变与缺血性脑卒中发病的相关性及其关联强度，为缺血性脑卒中高危人群的筛选及临床预防决策提供理论依据。方法：计算机检索Cochrane图书馆、EMbase、Pubmed三个英文数据库，检索中国生物医学文献数据库（CBM)、相关期刊论文（CNKI）、万方数据库三个中文数据库，并进行手动补充检索其他文献，检索时间为建库时间至2013年12月。建立严格统一的文献排除与纳入标准来筛选文献，对文献进行质量评价，提取数据，最后利用Revman5.2.0统计软件进行meta分析，并绘制森林图和漏斗图（用以评估发表偏倚）。结果： 1、MTHFR C677T等位基因分析结果初步筛查文章题目及摘要后得到文献22篇，其中英文7篇、中文15篇，根据纳入和排除标准最后得到英文3篇，中文11篇。经过meta分析可以得到I2=72%，说明存在实际的异质性，故采用随机性效应模型，在MTHFR C677T等位基因中，基因型(TT+TC)/CC的OR值为1.37，95%CI为0.98到1.92，p＜0.0001。2、CBS T833C等位基因分析结果根据纳入和排除标准初步筛查文章题目及摘要后得到文献19篇，其中英文文献7篇、中文文献12篇，最后得到英文文献2篇，中文文献7篇。进行Meta分析后可以得到I2=60%，说明存在实际的异质性，故采用随机性效应模型，在CBS T833C等位基因中，基因型（CC+CT）/TT的OR值为1.83，，95%CI为1.20到2.80，P=0.01。结论： 1、MTHFR C677T基因在MTHFR C677T等位基因中，与CC型基因对比，携带TC、TT基因型的人发生缺血性脑血管病风险明显增高。 2、CBS T833C基因在CBS T833C等位基因中，与TT型基因对比，携带CT、CC基因型的人发生缺血性脑血管病风险明显增高。
[Abstract]:Objective: Meta analysis was used to analyze the correlation between MTHFRN CBS gene and ischemic stroke, and to determine the correlation and intensity between MTHFR gene mutation and ischemic stroke. To provide theoretical basis for screening and clinical prevention decision of high risk population of ischemic stroke. Methods: Three English databases were searched by computer in Cochrane Library: Embase / Pubmed, Chinese Biomedical Literature Database (CBM), China Journal Full-text Database (CNKI), Wanfang Database (three Chinese databases), and other documents were retrieved manually. The retrieval time is from the time of building the database to December 2013. A strict and unified criterion of literature exclusion and inclusion was established to screen the literature, evaluate the quality of the literature, extract the data. Finally, the meta analysis was carried out by using Revman5.2.0 statistical software, and the forest map and funnel map were drawn (to evaluate the publication bias). Results: 1 result of allele analysis of MTHFR C677T After the title and summary of the preliminary screening articles, 22 papers were obtained, of which 7 were in English and 7 in English, 15 in Chinese, and 3 in English and 11 in Chinese according to the inclusion and exclusion criteria. After meta analysis, I _ 2 / 72 can be obtained, indicating the existence of actual heterogeneity. Therefore, the random effect model is used. In the MTHFR C677T allele, the OR value of genotype TTT TC)/CC is 1.37% 95 CI 0.98 to 1.92% P < 0.0001.2% CBS T833C allele analysis results. According to the criteria of inclusion and exclusion, 19 articles were selected, including 7 articles in Chinese and English, 12 in Chinese, 2 in English and 7 in Chinese. After Meta analysis, I _ 2 ~ (60) can be obtained, indicating the existence of actual heterogeneity. Therefore, the random effect model was used. In the CBS T833C allele, the OR value of CC CT)/TT was 1.83% 95 CI 1.20 to 2.80 P0. 01. Conclusion: 1. MTHFR C677T gene In the allele of MTHFR C677T, compared with CC genotype, the risk of ischemic cerebrovascular disease was significantly increased in people with TCT-TT genotype. CS-T833C gene In the allele of CBS T833C, compared with TT genotype, the risk of ischemic cerebrovascular disease was significantly increased in those with CTT833C genotype.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3

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