神经系统疾病患者血浆中BDV特异性CIC及BDV抗体的检测

发布时间：2018-04-27 10:20

本文选题：博尔纳病病毒 + 抗体　；参考：《遵义医学院》2014年硕士论文

【摘要】：目的：了解博尔纳病病毒（BDV）在贵州省部分地区人群中的的感染情况，探讨博尔纳病病毒与病毒性脑炎（VE）、多发性硬化（MS）、吉兰-巴雷综合征（GBS）以及帕金森病（PD）等神经系统疾病的相关性。方法：在2012年10月-2013年6月期间，收集遵义医学院附属医院、贵州省人民医院、贵阳医学院附属医院、贵阳市第二人民医院、贵阳中医学院第二附属医院等大型综合医院神经内科住院病人中，诊断为不明原因的病毒性脑炎（65例）、多发性硬化（7例）、吉兰-巴雷综合征（9例）、帕金森病（7例）外周血液样本，共计88例；另外收集遵义医学院附属医院体检科健康体检患者外周血液样本93例。并运用“三明治夹心”ELISA法，检测两组样本的BDV-CIC，并对阳性样本进行BDV抗体检测。结果：88例神经疾病组中有11例血浆中BDV-CIC为阳性，阳性率为12.6%（11/88）；93例对照组中BDV-CIC阳性7例，阳性率为7.5（7/93）；神经系统疾病组中病毒性脑炎BDV-CIC检出率为9.2%（6/65），与对照组差异无明显统计学意义（P0.05）；多发性硬化BDV-CIC检出率为28.6%（2/7），与对照组差异无明显统计学意义（P0.05）；吉兰-巴雷综合征BDV-CIC检出率为33.3%（3/9），与对照组差异有统计学意义（P0.05）；帕金森病BDV-CIC检测率为0%（0/7），，与对照组差异无明显统计学意义（P0.05）；神经系统疾病组BDV-CIC总体检出率高于健康对照组，但是差异无明显统计学意义（P0.05）；神经系统疾病组11例BDV-CIC阳性样本中BDV抗体阳性6例，双阳性率为6/11（54.5%），健康对照组7例BDV-CIC阳性样本中BDV抗体阳性5例，双阳性率为71.4%（5/7）。结论：贵州省部分地区人群中存在着BDV的感染；本次研究中病毒性脑炎、多发性硬化、吉兰-巴雷综合征患者中存在BDV的感染，BDV感染与吉兰-巴雷综合征的发生可能相关；与病毒性脑炎、多发性硬化、帕金森病的发生不一定相关。
[Abstract]:Objective: to investigate the infection of Borna disease virus (DVV) in some areas of Guizhou province. To investigate the correlation between Borna disease virus and viral encephalitis (VEC), multiple sclerosis (MS), Guillain-Barre syndrome (GBS) and Parkinson's disease (PD). Methods: from October 2012 to June 2013, affiliated hospitals of Zunyi Medical College, Guizhou Provincial people's Hospital, Guiyang Medical College affiliated Hospital, Guiyang second people's Hospital were collected. Among the inpatients in the Department of Neurology, the second affiliated Hospital of Guiyang College of traditional Chinese Medicine, and other large general hospitals, The peripheral blood samples of 65 cases of viral encephalitis diagnosed as unknown cause, 7 cases of multiple sclerosis, 9 cases of Guillain-Barre syndrome and 7 cases of Parkinson's disease) were collected. In addition, 93 samples of peripheral blood fluid were collected from healthy patients in Department of physical examination of affiliated Hospital of Zunyi Medical College. Using sandwich sandwich ELISA method, the BDV-CICs of two groups of samples were detected, and the positive samples were tested for BDV antibody. Results among 88 cases of neuropathy, 11 cases were positive for BDV-CIC in plasma, and the positive rate was 12. 6%. 7 cases were positive for BDV-CIC in the control group of 11% 88%. The positive rate of viral encephalitis in patients with nervous system diseases was 9.5% / 93%, the positive rate of viral encephalitis was 9.2 / 65%, which had no significant difference from the control group (P 0.05), the detection rate of multiple sclerosis BDV-CIC was 28.610 / 7% (P 0.05), and there was no significant difference between the two groups (P 0.05). The detection rate of BDV-CIC in syndrome was 33. 3 / 9. The difference was statistically significant compared with the control group (P 0. 05). The detection rate of BDV-CIC in Parkinson's disease was 0 / 7. There was no significant difference between the two groups (P 0. 05). The overall detection rate of BDV-CIC in patients with nervous system diseases was higher than that in healthy controls. But there was no significant difference in BDV antibody in 11 BDV-CIC positive samples, 6 cases were positive for BDV antibody, and the double positive rate was 6 / 1154.5%. In the healthy control group, there were 5 cases of BDV antibody positive and 71.4% / 7% positive rate of BDV antibody in 7 BDV-CIC positive samples. Conclusion: BDV infection may be associated with BDV infection in patients with viral encephalitis, multiple sclerosis and Guillain-Barre syndrome. It is not necessarily associated with viral encephalitis, multiple sclerosis, and Parkinson's disease.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R741

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