单唾液酸神经节苷脂注射液联合依达拉奉注射液对抗脑缺血性神经细胞损伤的作用

发布时间：2018-05-02 13:01

  本文选题：依达拉奉注射液 + 单唾液酸神经节苷脂注射液　； 参考：《中国临床药理学杂志》2017年11期

【摘要】：目的研究单唾液酸神经节苷脂联合依达拉奉对抗脑缺血性神经细胞损伤的作用。方法选取健康SD大鼠,按照体重随机分为模型组和实验组,每组21只。实验组:腹腔注射单唾液酸神经节苷脂20 mg·kg~(-1),每天1次;腹腔注射依达拉奉3 mg·kg~(-1),每天2次。模型组,腹腔注射等量1%的0.9%Na Cl。均给药14 d。用大脑中动脉阻塞线栓法制作局灶性脑缺血再灌注动物模型。用图像分析系统,依据大鼠脑缺血半暗袋定位,在相同的视野下,将大脑中动脉阻塞的同侧额顶叶皮质上部界定为半暗带的等值观察区,用免疫组化法对大鼠丝氨酸-苏氨酸蛋白激酶(Akt)、糖原合酶激酶-3(GSK-3β)、3-磷酸肌醇依赖性蛋白激酶(PDK1)及磷脂酰肌醇3激酶(PI3K)的蛋白免疫反应阳性细胞平均光密度与阳性面积单位进行对比分析。结果模型组和实验组PI3K蛋白、Akt蛋白及PDK1蛋白的表达均加强、而GSK3β蛋白表达均减弱。7 d后,PI3K蛋白、Akt蛋白、PDK1蛋白及GSK3β蛋白的阳性单位面积,模型组与实验组分别为(19.58±1.13),(26.91±0.90)mm~2;(15.98±0.48),(22.87±1.20)mm~2;(17.97±0.58),(26.02±0.54)mm~2;(22.03±0.92),(14.02±0.45)mm~2,组间对比差异均有统计学意义(均P0.05)。7 d后这4种蛋白的平均光密度值,模型组与实验组分别为0.19±0.01,0.28±0.02;0.16±0.01,0.25±0.01;0.16±0.01,0.25±0.01;0.22±0.01,0.14±0.01,组间对比差异均有统计学意义(均P0.05)。结论单唾液酸神经节苷脂结合依达拉奉可抵抗脑缺血区域神经细胞的损伤。
[Abstract]:Objective to study the effect of monoglycoside combined with Edaravone on cerebral ischemic nerve cell injury. Methods healthy Sprague-Dawley rats were randomly divided into model group and experimental group according to body weight with 21 rats in each group. Experimental group: single sialic acid ganglioside was injected intraperitoneally, once a day, and Edaravone 3 mg / kg, twice a day. In the model group, 1% 0.9%Na Cl was injected intraperitoneally. The drug was given for 14 days. The animal model of focal cerebral ischemia reperfusion was established by middle cerebral artery occlusion. Using an image analysis system, the middle cerebral artery occlusion of the upper frontal parietal cortex of the middle cerebral artery was defined as the equivalent observation area of the penumbra under the same field of vision according to the localization of the cerebral ischemic semi-dark bag in rats. The average optical density and positive surface of protein immunoreactive cells of rat serine threonine protein kinase, glycogen synthase kinase GSK-3 尾 -phosphoinositol dependent protein kinase PDK1 and phosphatidylinositol 3 kinase PI3K were determined by immunohistochemical method. The unit of product is compared and analyzed. Results the expression of PI3K protein and PDK1 protein were enhanced in model group and experimental group, while the expression of GSK3 尾 protein was decreased at 7. 7 d after the expression of GSK3 尾 protein, the positive unit area of PDK1 protein and GSK3 尾 protein of PI3K protein was decreased. 妯″瀷缁勪笌瀹為獙缁勫垎鍒�涓�(19.58卤1.13),(26.91卤0.90)mm~2;(15.98卤0.48),(22.87卤1.20)mm~2;(17.97卤0.58),(26.02卤0.54)mm~2;(22.03卤0.92),(14.02卤0.45)mm~2,缁勯棿瀵规瘮宸�寮傚潎鏈夌粺璁″�︽剰涔，

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