骨髓间充质干细胞移植对大鼠癫痫海马神经炎症的抑制作用研究

发布时间：2018-05-15 07:27

本文选题：脑炎 + 骨髓　；参考：《中华细胞与干细胞杂志(电子版)》2016年06期

【摘要】：目的探讨骨髓间充质干细胞(BMSCs)移植对大鼠癫痫海马神经炎症的抑制作用。方法体外分离纯化SD大鼠BMSCs,BMSCs处理的无血清αMEM和单纯无血清αMEM分别设为实验组和对照组,而后应用ELISA检测BMSCs培养基中抗炎细胞因子单核细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α-刺激基因-6(TSG-6)的表达。匹罗卡品腹腔注射诱导大鼠癫痫模型,侧脑室注射5×10~6个BMSCs和同体积生理盐水分别设为实验组和对照组,未经处理的SD大鼠设为正常对照,4 d后免疫组织化学检测各组海马小胶质细胞或活化的小胶质细胞表达变化。单因素方差分析检测各组数据差异,组间数据比较采用独立t检验。结果 BMSCs条件培养基中MCP-1(61.8±15.64)pg/ml和TSG-6(1.3±0.12)ng/ml的表达较对照组明显上升(P0.01)。匹罗卡品诱导癫痫模型后,小胶质细胞胞体和突起所占面积百分比(39.2%±7.68%)较正常对照组(11.7%±3.47%)明显增多(P0.01),且ED1染色发现小胶质细胞明显活化。BMSCs移植4 d后,小胶质细胞和活化的小胶质细胞表达较癫痫对照组明显下降(P0.01)。结论 BMSCs具有旁分泌抗炎细胞因子的潜能,其移植对大鼠癫痫海马神经炎症具有明显抑制作用。
[Abstract]:Objective to investigate the inhibitory effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on hippocampal neuroinflammation in rats. Methods SD rat BMSCs was isolated and purified in vitro. The serum free alpha MEM and serum free alpha MEM treated by BMSCs were set up as experimental and control groups respectively. Then ELISA was used to detect the chemotaxis of anti inflammatory cytokine monocyte in BMSCs medium. Protein -1 (MCP-1) and tumor necrosis factor - alpha stimulation gene -6 (TSG-6) expression. Pilocarpine intraperitoneal injection induced rat epilepsy model, lateral ventricle injection of 5 x 10~6 BMSCs and the same volume of normal saline were set as experimental group and control group respectively. The untreated SD rats were set as normal controls. After 4 D, immunohistochemistry was used to detect hippocampal small glue. The difference of data was detected by single factor analysis of variance, and the data were compared by independent t test. Results the expression of MCP-1 (61.8 + 15.64) pg/ml and TSG-6 (1.3 + 0.12) ng/ml in BMSCs conditioned medium was significantly higher than that in the control group (P0.01). Microglia was microglia after pilocarpine induced epileptic model The percentage of cell body and protuberance (39.2% + 7.68%) was significantly increased (P0.01) than that in normal control group (11.7% + 3.47%), and ED1 staining showed that microglia cells obviously activated.BMSCs transplantation for 4 D, and the expression of microglia and activated microglia decreased significantly compared with those in the epilepsy control group (P0.01). Conclusion BMSCs has paracrine anti-inflammatory cells. Its potential transplantation has a significant inhibitory effect on hippocampal neuritis in rats.

【作者单位】：西安市中心医院神经外科;
【基金】：陕西省科学技术研究发展计划项目(2014KJXX-29)
【分类号】：R742.1

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