BDNF和β干扰素对星形胶质细胞增殖及对EAE大鼠免疫因子的影响

发布时间：2018-05-24 23:43

本文选题：脑源性神经生长因子 + β干扰素　；参考：《现代免疫学》2017年06期

【摘要】：研究脑源性神经生长因子(brain derived neurotrophic factor,BDNF)对正常SD大鼠和实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)SD大鼠中星形胶质细胞生长的影响,以及BDNF和β干扰素(Beta interferon,IFN-β)对EAE SD大鼠免疫因子的影响,为临床治疗多发性硬化症(multiple sclerosis,MS)提供更多理论依据。以不完全弗氏佐剂和灭活结核分枝杆菌诱导EAE动物模型,培养健康对照组和EAE实验组新生大鼠的星形胶质细胞,传代后向其培养液中加入BDNF(0ng/mL、0.1ng/mL、1ng/mL、10ng/mL)和IFN-β(0μg/mL、0.01μg/mL、0.1μg/mL、1μg/mL),通过免疫荧光观察星形胶质细胞数量的变化(喁±s),通过ELISA检测吸光度[D(450nm)]并通过统计分析评估抗炎因子IL-10和CXCL1以及促炎因子IL-1和TNF-α的水平。本实验发现实验组和对照组的培养液中加入一定浓度BDNF后其星形胶质细胞数量增加,并且随着BDNF浓度逐渐增加,星形胶质细胞数量也逐渐增加(喁±s)。在实验组中,一定浓度范围内的BDNF(0ng/mL、0.1ng/mL、1ng/mL、10ng/mL)和IFN-β(0μg/mL、0.01μg/mL、0.1μg/mL、1μg/mL)能够提高IL-10和CXCL1的水平(P0.001),同时降低IL-1和TNF-α的水平(P0.001)。本研究表明BDNF对星形胶质细胞的增殖有促进作用,BDNF和IFN-β有一定的抗炎作用,对MS有治疗价值。
[Abstract]:To study the effects of brain-derived neurotrophic factor (BDNF) on the growth of astrocytes in normal SD rats and experimental autoimmune encephalomyelitis rats, and the effects of BDNF and 尾 interferon beta-interferonsis-IFN- 尾 on the immune factors of EAE SD rats. To provide more theoretical basis for clinical treatment of multiple sclerosis. EAE animal model was induced by incomplete Freund's adjuvant and inactivated Mycobacterium tuberculosis. Astrocytes of neonatal rats in healthy control group and EAE experimental group were cultured. After passage, we added BDNF0ngmL0. 1ngmL0. 1ngmL10 ngmL) and IFN- 尾 0 渭 g / mL0. 01 渭 g / mL0. 1 渭 g / mL1 渭 g / mL. We observed the changes of astrocyte count by immunofluorescence. We observed the changes of astrocyte count by immunofluorescence, and evaluated the levels of anti inflammatory factor IL-10 and CXCL1, IL-1 and TNF- 伪 by ELISA detection [DN450nmL] and analyzed the level of anti inflammatory factor IL-10 and CXCL1 by statistical analysis. The results were as follows: 1 渭 g / mL1 渭 g / mLnL1 渭 g / mLnL. the levels of anti inflammatory factor IL-10 and CXCL1, IL-1 and TNF- 伪 were evaluated by statistical analysis. It was found that the number of astrocytes increased with the concentration of BDNF in the culture medium of experimental group and control group, and the number of astrocytes increased with the increase of BDNF concentration. In the experimental group, in a certain concentration range, BDNF0ngmL0. 1 ng / mLnL1) and IFN- 尾 0 渭 g / mL0. 01 渭 g / mL1 渭 g / mL) increased the levels of IL-10 and CXCL1, while reducing the levels of IL-1 and TNF- 伪. This study suggests that BDNF can promote the proliferation of astrocytes. BDNF and IFN- 尾 have some anti-inflammatory effects and have therapeutic value to MS.
【作者单位】：大理市第一人民医院神经内科;昆明医科大学第一附属医院神经内科;昆明医科大学第一附属医院精神科;
【基金】：云南省科技厅自然科学基金应用基础研究项目(2013FB146)
【分类号】：R744.51

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