半胱氨酸组织蛋白酶S基因敲除对高岭土诱导小鼠交通性脑积水的影响

发布时间：2018-05-25 00:02

本文选题：脑积水 + 高岭土　；参考：《上海交通大学学报(医学版)》2017年10期

【摘要】：目的·观察半胱氨酸组织蛋白酶S(CatS)基因敲除对高岭土诱导小鼠交通性脑积水严重程度的影响。方法·针对8只CatS基因敲除(CatS~(-/-))小鼠和16只野生型(WT)C57BL/6小鼠,通过枕大池注射高岭土混悬液的方法建立交通性脑积水模型,建模前及建模后1周利用头颅磁共振(MRI)扫描测量小鼠侧脑室体积并计算侧脑室指数,比较2组小鼠脑积水严重程度。结果·建模后1周CatS~(-/-)组死亡1只(12.5%),WT组死亡2只(12.5%),2组间死亡率的差异无统计学意义(P=1.000);头颅MRI结果显示存活CatS~(-/-)组和WT组小鼠均有不同程度脑室扩大,建模前CatS~(-/-)组(n=8)侧脑室指数为0.05±0.01,WT组(n=16)侧脑室指数为0.04±0.01(P=0.720)。建模后1周CatS~(-/-)组(n=7)侧脑室指数为0.13±0.02,其中侧脑室指数增加1倍以上者5只,占71.4%;WT组(n=14)侧脑室指数为0.11±0.01,侧脑室指数增加1倍以上者10只,占71.4%;2组比较,差异无统计学意义(P=0.950)。结论·枕大池注射高岭土混悬液后1周,小鼠侧脑室明显扩大伴脑积水发生,模型成功率高;CatS基因敲除对高岭土诱导小鼠脑交通性脑积水发展无明显影响。
[Abstract]:Objective to observe the effect of cysteine cathepsin Sch CatS knockout on the severity of communicating hydrocephalus induced by kaolin in mice. Methods the model of communicating hydrocephalus was established by injecting kaolin suspension into occipital cistern in 8 CatS knockout mice and 16 wild type WTA C57BL / 6 mice. The volume of lateral ventricle was measured and the index of lateral ventricle was calculated before and 1 week after modeling. The severity of hydrocephalus was compared between the two groups. Results one week after modeling, there was no significant difference in the mortality rate between the two groups in the WT group and the WT group, but there was no significant difference in the mortality rate between the two groups, and the cranial MRI results showed that both the surviving CatS group and WT group had different degrees of ventricular dilatation, and the results showed that there was no significant difference between the two groups in the mortality of the two groups, and the results of cranial MRI showed that both the survival group and the WT group had different degrees of ventricular dilatation. Before modeling, the ventricular index (VI) was 0.05 卤0.01kW / L) in the Catson-r-R group (0.04 卤0.01P0. 720) in WT group (P = 0. 720) and 0. 05 卤0. 01 (P = 0. 720) in the control group (n = 0. 05 卤0. 01). One week after modeling, the index of lateral ventricle was 0.13 卤0.02in the CatSti-r-R group (0.13 卤0.02), in which the index of lateral ventricle increased more than twice in 5 rats, accounting for 71.4% of WT group (0.11 卤0.01), while the index of lateral ventricle increased more than 1 fold in 10 rats (71.4%). There was no significant difference between the two groups (P = 0.950). Conclusion 1 week after injection of kaolinite suspension into cistern, the lateral ventricle was obviously enlarged with hydrocephalus in mice. The high success rate of CatS gene knockout had no significant effect on the development of cerebral communicating hydrocephalus induced by kaolin in mice.
【作者单位】：上海交通大学医学院附属第九人民医院神经内科;华东师范大学物理系上海磁共振重点实验室;浙江中医药大学附属杭州市西溪医院神经内科;
【基金】：上海市科学技术委员会创新行动计划基础研究重点项目(14JC1404300);上海市科学技术委员会生物医药支撑项目(16411953100) 上海交通大学医学院多中心临床研究项目(DLY201614) 上海交通大学医学院—中国科学院神经科学研究所脑疾病临床研究中心2015年率先启动项目(2015NKX006) 上海申康医院发展中心临床科技创新项目(SHDC12015310)~~
【分类号】：R742.7

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