miR-425对胶质瘤细胞迁移与侵袭的影响

发布时间：2018-05-26 00:46

本文选题：胶质瘤 + miR-　；参考：《中国老年学杂志》2017年07期

【摘要】：目的探讨miR-425对人胶质细胞瘤U87细胞迁移与侵袭能力的影响。方法利用Lipo2000转染试剂体外瞬时转染人胶质细胞瘤U87,将细胞分为实验组(转染miR-425 mimics)、抑制组(转染miR-425 inhibitors)、空白组(无转染)。Real-time PCR检测细胞中miR-425表达;划痕实验检测细胞的迁移距离,Transwell实验检测细胞的迁移以及侵袭能力,Western印迹检测基质金属蛋白(MMP)2、MMP9的表达水平。结果与空白组相比,miR-425 mimics组miR-425表达明显上调,约为空白组的8倍,细胞侵袭与迁移能力下降,MMP2、MMP9表达下降,miR-425 inhibitors组miR-425表达明显下调,约为空白组的0.14倍,细胞侵袭与迁移能力显著增强,MMP2、MMP9表达增强。结论 miR-425的表达水平与胶质瘤U87细胞的迁移与侵袭能力密切相关。
[Abstract]:Objective to investigate the effect of miR-425 on migration and invasion of human glioma U87 cells. Methods Human glioma U87 cells were transiently transfected with Lipo2000 transfection reagent in vitro. The cells were divided into experimental group (transfected with miR-425 mimicsl), inhibition group (transfected with miR-425 inhibitor) and blank group (without transfection. Real-time PCR was used to detect the expression of miR-425 in the cells. The migration distance was detected by scratch assay and the migration was detected by Transwell assay, and the expression of matrix metalloprotein MMP2 and MMP9 was detected by Western blotting. Results compared with the control group, the expression of miR-425 in the mimics group was significantly up-regulated, which was about 8 times higher than that in the blank group. The cell invasion and migration ability was decreased. The expression of MMP2MMP9 in the inhibitors group was down-regulated significantly, which was about 0.14 times of that in the blank group. The ability of cell invasion and migration significantly enhanced the expression of MMP2 and MMP9. Conclusion the expression of miR-425 is closely related to the migration and invasion of U87 cells.
【作者单位】：甘肃省人民医院干部病房神经内科;云南省第二人民医院神经内科;
【分类号】：R739.41

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