MMP-9在颅内动脉瘤组织中的表达变化及其对血管平滑肌细胞凋亡的影响

发布时间：2018-05-30 22:42

本文选题：颅内动脉瘤 + 基质金属蛋白酶　；参考：《山东医药》2017年28期

【摘要】：目的观察基质金属蛋白酶9(MMP-9)在颅内动脉瘤组织中的表达变化及其对血管平滑肌细胞凋亡的影响。方法收集新鲜颅内动脉瘤组织20例份、正常颅内动脉血管组织10例份,采用Western blotting法检测MMP-9蛋白表达。分离培养颅内动脉瘤血管平滑肌细胞,随机分为MMP-9 siRNA组、siRNA对照组、空白对照组,分别加入MMP-9 siRNA、对照序列siRNA、转染试剂培养48 h,采用Western blotting法检测各组MMP-9及Bcl-2、Bax、p65、p-p65蛋白表达,流式细胞术检测各组细胞凋亡率。结果颅内动脉瘤组织MMP-9蛋白相对表达量明显高于正常颅内动脉血管组织(P0.01)。与空白对照组、siRNA对照组比较,MMP-9 siRNA组MMP-9及Bax、p-p65蛋白相对表达量降低,Bcl-2蛋白相对表达量升高(P均0.01),p65蛋白相对表达量未见明显变化(P0.05),而siRNA对照组与空白对照组各指标比较P均0.05。MMP-9 siRNA组细胞凋亡率明显低于siRNA对照组、空白对照组(P均0.01),而siRNA对照组与空白对照组比较P0.05。结论 MMP-9在颅内动脉瘤组织中呈高表达。下调MMP-9蛋白表达能抑制颅内动脉瘤血管平滑肌细胞凋亡,其机制可能与NF-κB信号通路活性降低有关。
[Abstract]:Objective to observe the expression of matrix metalloproteinase 9 (MMP-9) in intracranial aneurysms and its effect on apoptosis of vascular smooth muscle cells. Methods 20 cases of fresh intracranial aneurysms and 10 cases of normal intracranial artery tissues were collected and the expression of MMP-9 protein was detected by Western blotting method. The vascular smooth muscle cells of intracranial aneurysms were isolated and cultured. They were randomly divided into two groups: MMP-9 siRNA group (control group) and blank control group (control group). The expression of MMP-9 and Bcl-2Baxp65 p65 protein were detected by Western blotting assay after 48 h culture with transfection reagent. Apoptosis rate was detected by flow cytometry. Results the relative expression of MMP-9 protein in intracranial aneurysms was significantly higher than that in normal intracranial artery tissues (P 0.01). Compared with the control group of blank control group, the relative expression of MMP-9 and Bax-p65 protein in MMP-9 siRNA group decreased the relative expression of Bcl-2 protein increased (P < 0.01), and the relative expression of p65 protein in siRNA control group was not significantly changed, while that in siRNA control group was higher than that in blank control group (P < 0.01), but the relative expression of Bax-p65 protein in siRNA control group was higher than that in blank control group (P < 0.05). The apoptosis rate in 0.05.MMP-9 siRNA group was significantly lower than that in siRNA control group. P of the blank control group was 0.01, while that of the siRNA control group was higher than that of the blank control group (P 0.05). Conclusion MMP-9 is highly expressed in intracranial aneurysms. Down-regulating the expression of MMP-9 protein can inhibit the apoptosis of vascular smooth muscle cells in intracranial aneurysms, which may be related to the decrease of NF- 魏 B signaling pathway activity.
【作者单位】：德州市市立医院;
【分类号】：R743

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