丁苯酞对缺血脑组织RGMa表达及轴索损伤的影响

发布时间：2018-06-06 01:04

本文选题：丁苯酞 + 脑缺血　；参考：《放射学实践》2017年10期

【摘要】：目的:探讨丁苯酞对缺血性脑损伤后缺血脑组织排斥导向分子(RGMa)表达的影响。方法:120只成年雄性SD大鼠,随机分为假手术组,大脑中动脉闭塞(MCAO)模型组,生理盐水组,丁苯酞干预组。选取缺血后1h、3h、6h、12h、24h,进行神经功能评分,免疫组织化学观察RGMa的表达情况。结果:MCAO模型组、生理盐水组、丁苯酞组的改良的神经功能评分(mNSS)均高于假手术组,且随着时间的延长,mNSS评分越高;在缺血后3h、6h、12h、24h时,丁苯酞组的mNSS评分较MCAO模型组降低,差异具有统计学意义(P0.05)。MCAO模型组、生理盐水组大鼠缺血后,不同时间点缺血区的RGMa蛋白阳性表达均有所增加,呈棕黄色染色。局灶性脑缺血后RGMa蛋白表达逐渐增多,在12h表达量最多;丁苯酞组在相应时间点的RGMa蛋白的表达较MCAO模型组有所降低,在6h、12h、24h时间点差异具有统计学意义。结论:丁苯酞可减少缺血性脑损伤大鼠的缺血脑组织的RGMa的表达,从而发挥神经功能保护作用。
[Abstract]:Aim: to investigate the effect of butyphthalide on the expression of rejection oriented molecule (RGMa) in ischemic brain tissue after ischemic brain injury. Methods 120 adult male Sprague-Dawley rats were randomly divided into sham-operation group, MCAO model group, normal saline group and butyrophthalide intervention group. The nerve function score and the expression of RGMa were observed by immunohistochemical method in 1 hour, 3 h, 6 h and 12 h and 24 h after ischemia. Results the modified neural function scores in the MCAO model group, normal saline group and butyphthalide group were higher than those in the sham operation group, and the higher the score was with the prolongation of time, the lower the mNSS score in the butyphthalide group was compared with that in the MCAO model group at 3 h, 6 h, 12 h and 24 h after ischemia. The difference was statistically significant in the P0.05-MCAO model group and in the saline group. After ischemia, the positive expression of RGMa protein increased at different time points, and showed brown staining. The expression of RGMa protein increased gradually after focal cerebral ischemia, and the expression of RGMa protein in butyphthalide group at the corresponding time point was lower than that in the MCAO model group, and the difference was statistically significant at the time point of 6 h and 12 h and 24 h after focal cerebral ischemia, and the expression of RGMa protein in butyphthalide group was lower than that in the MCAO model group. Conclusion: butyphthalide can reduce the expression of RGMa in ischemic brain tissue of rats with ischemic brain injury, thus exerting neuroprotective effect.
【作者单位】：郑州大学第一附属医院磁共振科;
【基金】：2014年国家自然科学基金项目(U1404823)
【分类号】：R743.31

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