聚焦超声对斑马鱼神经胶质瘤模型的治疗研究

发布时间：2018-06-15 07:01

本文选题：聚焦超声 + 高强度聚焦超声　；参考：《重庆医科大学学报》2015年01期

【摘要】：目的:通过研究聚焦超声对斑马鱼神经胶质瘤模型治疗后肿瘤组织和肿瘤血管的变化,探讨其对神经胶质瘤及肿瘤血管的影响。方法:将72只转基因斑马鱼(fli:GFP)随机分为3组,每组24只,分别是对照组(Fli组)、肿瘤组(U87组)、治疗组(U87+HIFU组)。在受精2 d后,对肿瘤组和治疗组注射神经胶质瘤细胞(U87-RFP),注射1 d后对治疗组进行聚焦超声治疗。在治疗后连续2 d用荧光显微镜观察3组斑马鱼的肿瘤大小及肿瘤血管增生情况,q PCR法检测血管内皮生长因子(vascular endothelial growth factor,VEGF)Aa和血管内皮生长因子受体(vascular endothelial growth factor receptor,VEGFR)2的m RNA的表达情况。结果:U87组斑马鱼卵黄内有大量神经胶质瘤细胞且肿瘤血管明显增生,VEGF Aa和VEGFR2的m RNA表达升高(VEGF Aa:Fli:1.01±0.13 vs.U87:1.45±0.10,P=0.002;VEGFR2:Fli:1.01±0.12 vs.U87:1.41±0.07,P=0.001);经聚焦超声治疗后,斑马鱼卵黄内的神经胶质瘤细胞显著减少,血管增生不明显,VEGF Aa和VEGFR2的m RNA表达也较U87组降低(VEGF Aa:U87+HIFU:1.14±0.05 vs.U87:1.45±0.10,P=0.009;VEGFR2:U87+HIFU:1.16±0.06 vs.U87:1.41±0.07,P=0.012)。结论:聚焦超声能有效杀灭神经胶质瘤细胞,并且能减少肿瘤血管的生成,对治疗神经胶质瘤具有积极作用。
[Abstract]:Aim: to study the changes of tumor tissue and tumor vessels after treatment of zebrafish glioma model by focused ultrasound, and to investigate the effect of focused ultrasound on glioma and tumor vessels. Methods: 72 transgenic zebrafish flivre GFPs were randomly divided into 3 groups: control group (Fli group), tumor group (U87) and treatment group (U87 HIFU). After 2 days of fertilization, the tumor group and the treatment group were injected with U87-RFPX, and the treatment group was treated with focused ultrasound 1 day later. The tumor size and tumor angiogenesis of zebrafish in three groups were observed by fluorescence microscope for 2 days after treatment. The expression of vascular endothelial growth factor (endothelial growth) and vascular endothelial growth factor receptor (VEGFR) A and vascular endothelial growth factor receptor (VEGFR) were detected by q-PCR. Results there were a large number of glioma cells in the yolk of zebrafish, and the expression of VEGF AA and VEGFR2 mRNA was increased in the zebrafish egg yolk. The expression of VEGF a: Fli1: 0.13 vs.U871.45 卤0.10P0. 002VEGFR2: Fliw 1.01 卤0.12 vs.U871.41 卤0.07P0. 001. After focused ultrasound treatment, the number of glioma cells in zebrafish egg yolk decreased significantly. The mRNA expression of VEGF Aa and VEGFR2 in vascular hyperplasia group was also lower than that in U87 group. The mRNA expression of VEGF A: a: U87 HIFU: 1.14 卤0.05 vs.U8710: 1.45 卤0.10p0. 009 + VEGFR2: U87 HIFUU: 1.16 卤0.06vs.U871.41 卤0.07PU 0.012. Conclusion: focused ultrasound can effectively kill glioma cells, reduce tumor angiogenesis, and play an active role in the treatment of glioma.
【作者单位】：重庆医科大学基础医学院组织胚胎学教研室;重庆医科大学生物医学工程学院省部共建超声医学工程国家重点实验室超声医学工程重庆市市级重点实验室;重庆医科大学基础医学院发育生物学研究室;
【分类号】：R739.41;R-332

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