女性激素与海马组织代谢物间的相关性研究

发布时间：2018-06-21 23:51

本文选题：围绝经期 + 雌二醇　；参考：《广州中医药大学》2017年硕士论文

【摘要】：目的:近年来我国老龄化现象日益加重,每年约有几十万的女性经历围绝经期,女性围绝经期症状主要是记忆力减退、学习能力下降、抑郁、焦虑等,且围绝经期主要生理变化是性激素水平的波动。海马是大脑中涉及学习和记忆的功能区域,在中枢神经退行性疾病的发生与发展中起到重要的作用。磁共振波谱学是磁共振检查技术中的生化代谢分析,是非侵入性测定活体组织中相应化合物组成成分及其含量的影像学检测技术,因组织的生物学改变常常先于形态学改变,所以它在分析和探讨女性不同性激素水平与海马区细胞代谢物之间的联系作用方面具有不可替代的应用价值。因此,全面研究不同血清性激素水平与海马组织代谢物的联系变化规律,有助于为累及海马的相关疾病如衰老、Alzheimer病等的鉴别与早期诊断提供可靠的影像学参考依据。方法:选取围绝经期女性31例(围绝经期组)、健康年轻女性31例(健康年轻组),均收集年龄、体重、身高等基本信息;选用3.0T磁共振氢质子波谱技术采集所有志愿者大脑半球双侧海马(与学习和记忆相关的区域)的代谢物即N-乙酰天冬氨酸(NAA)、总肌酸(Cr)、胆碱(Cho)的浓度,以及测定每位女性血清雌二醇、促黄体生成素、促卵泡刺激素、孕酮、睾酮水平。资料均采用独立样本t检验、Pearson相关进行统计学分析。成果:围绝经期组和健康年轻组相比,血清雌二醇水平显著降低(P=0.000);血清促卵泡刺激素水平显著降低(P=0.012);血清睾酮水平显著降低(P=0.000);右侧海马NAA/Cr显著降低(P=0.013),右侧海马NAA/(Cr+Cho)显著降低(P=0.006)。经配对t检验,围绝经期组女性海马NAA/Cr左右侧具有显著差异(P=0.010);围绝经期组女性海马NAA/(Cr+Cho)左右侧具有显著差异(P=0.004)。健康年轻组女性海马NAA/Cr左右侧具有显著差异(P=0.012),健康年轻组女性海马NAA/(Cr+Cho)左右侧具有显著差异(P=0.002)。Pearson相关分析表明,健康年轻组女性血清雌二醇水平与左侧海马NAA/Cr、左侧NAA/(Cr+Cho)、右侧海马NAA/Cr、右侧NAA/(Cr+Cho)之间存在正相关(P=0.001、P=0.000、P=0.022、P=0.034)。围绝经期组女性血清促黄体生成素水平与左侧海马NAA/Cr、左侧海马Cho/Cr、左侧海马NAA/(Cr+Cho)、右侧海马 NAA/(Cr+Cho)之间存在负相关(P=0.007、P=0.044、P=0.014、P=0.028);围绝经期组女性血清睾酮与右侧海马Cho/Cr之间存在正相关(P=0.027);围绝经期组女性年龄与右侧海马NAA/Cr之间存在负相关(P=0.047)。结论:健康年轻女性血清雌二醇水平越高,海马左右侧NAA/Cr、NAA/(Cr+Cho)越高;围绝经期女性血清促黄体生成素水平越高,海马左侧NAA/Cr、海马左右侧NAA/(Cr+Cho)越低;女性血清雌二醇、促黄体生成素等可能通过某种机制影响了海马的代谢。
[Abstract]:Objective: in recent years, the aging phenomenon in China has become more and more serious. Hundreds of thousands of women experience peri-menopausal period every year. The symptoms of menopause are mainly memory loss, learning ability decline, depression, anxiety, etc. The main physiological changes in the perimenopausal period were the fluctuation of sex hormone levels. Hippocampus is a functional region of the brain involved in learning and memory, which plays an important role in the occurrence and development of central neurodegenerative diseases. Magnetic Resonance Spectroscopy (MRS) is a biochemical metabolic analysis in MRI. It is a non-invasive imaging technique for the determination of components and contents of corresponding compounds in living tissues, because the biological changes of tissues often precede morphological changes. Therefore, it has an irreplaceable application value in analyzing and discussing the relationship between different sex hormone levels and cellular metabolites in hippocampal area. Therefore, a comprehensive study of the relationship between serum levels of sex hormones and metabolites in hippocampal tissues is helpful to provide a reliable imaging reference for the differential diagnosis and early diagnosis of diseases related to the hippocampus, such as Alzheimer's disease. Methods: 31 cases of perimenopausal women (perimenopausal group) and 31 healthy young women (healthy young group) were selected. The basic information of age, weight and height were collected. The concentrations of N-acetylaspartate (NAA), total creatine (Cr) and choline (Cho) in the bilateral hippocampus (learning and memory-related regions) of all volunteers were collected by 3.0T magnetic resonance proton spectroscopy. Serum estradiol, luteinizing hormone, follicle stimulating hormone, progesterone and testosterone were measured. The data were statistically analyzed by independent t-test and Pearson correlation. Results: serum estradiol level (P0. 000), serum follicle stimulating hormone level (P0. 012), serum testosterone level (P0. 000), NAA / Cr (P0. 013) in the right hippocampus and NAA / (Cr Cho) in the right hippocampus were significantly decreased (P0. 006). By paired t test, there was a significant difference in NAA / (Cr Cho) between the left and right sides of hippocampus in the perimenopausal group (P0. 010), and in the peri-menopausal group (P0. 004), there was a significant difference between the left and right sides of NAA / (Cr Cho) in the hippocampus of the perimenopausal women. NAA / (Cr Cho) in hippocampus of healthy young women showed significant difference (P0. 012). Pearson correlation analysis showed that NAA / (Cr Cho) in healthy young women had significant difference (P0. 002). Pearson correlation analysis showed that NAA / (Cr Cho) in healthy young women had significant difference (P < 0. 002). The serum estradiol levels in healthy young women were positively correlated with NAA / Cr, NAA / (Cr Cho) in the left hippocampus, NAA / (Cr Cho) in the right hippocampus and NAA / (Cr Cho) in the right hippocampus. There was a negative correlation between serum luteinizing hormone levels and NAA / Cr, Chor / Cr, NAA / (Cr Cho) in left hippocampus, NAA / (Cr Cho) in left hippocampus and NAA / (Cr Cho) in right hippocampus in perimenopausal women (P0. 007, P0. 044, P0. 014, P0. 028), and there was a relationship between serum testosterone and right hippocampal Chor / Cr in perimenopausal women. There was a positive correlation (P0. 027) and a negative correlation (P0. 047) between age and NAA / Cr in the right hippocampus in the perimenopausal group. Conclusion: the higher the serum estradiol level in healthy young women, the higher NAA / Cr Cho / (Cr Cho) in left and right hippocampus, the higher the serum luteinizing hormone level in menopausal women, the lower NAA / Cr Cho in left hippocampus and left side of hippocampus, and the lower in serum estradiol in female. Luteinizing hormone may influence the metabolism of hippocampus through some mechanism.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R741

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