Smac和XIAP在人脑胶质瘤组织中的表达及意义

发布时间：2018-06-24 21:15

本文选题：胶质瘤 + 第二个线粒体衍生的半胱氨酸蛋白酶激活剂　；参考：《兰州大学》2014年硕士论文

【摘要】：目的胶质瘤与其它颅内肿瘤比较所占比例最高,经过外科干预后仍无法有效的切除干净,导致术后容易复发,预后差,胶质瘤具有着高发病率、高复发率、高病死率和低治愈率的三高一低的特点。本课题旨在通过免疫组化的方法检测研究半胱氨酸蛋白酶的第二个线粒体激活因子(the second mitochodria-derived activator of caspase, Smac)与X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)在神经胶质瘤中的表达情况,分析二者之间的相互作用关系,研究Smac、XIAP与神经胶质瘤恶性程度之间的关系,为能够准确的预判胶质瘤的恶性程度和预后给予一定帮助,并能从凋亡角度考虑肿瘤的发生。方法自2011年06月至2013年12月期间在兰州大学第二医院神经外科手术切除的62例胶质瘤标本。采用标准,根据WHO(2007年)的分类和分级标准。免疫组织化学采用链霉素抗生物-过氧化物酶法(SP法)检测,空白对照用磷酸缓冲液代替一抗。Smac与XIAP蛋白阳性表达为细胞浆中出现棕黄色颗粒。免疫组化结果计分根据标准免疫反应积分法,观察阳性细胞染色深浅并对光学显微镜视野内的阳性细胞进行计数,并计算阳性细胞数占细胞总数的百分比。(阳性细胞百分比：未发现阳性细胞的为0分(一),阳性细胞数占细胞总数的10%以下为1分,11～25%为2分,25～50%为3分,50%以上为4分,根据流行的染色程度(没有着色是0分,淡黄色是1分,棕黄色是2分,棕褐色是3分)。免疫组化染色评分(免疫组化积分=阳性细胞百分比PP×染色强度SI)由0-12这13个分级组成,没有表达(一)是0分,弱阳性表达(+)是1到4分,阳性表达(++)是5到8分,强阳性表达(+++)是9到12分,根据实验记录数据用统计软件SPSS18.0分析两者的表达情况及与临床资料的相关性。结果62例人脑胶质瘤标本中,Smac蛋白在低级别胶质瘤和高级别胶质瘤中的阳性表达率分别为89.66%,78.79%,差别(P=0.000)具有统计学意义,与患者病理组织类型、性别及年龄无关。XIAP蛋白在低级别胶质瘤和高级别胶质瘤中的阳性表达率分别为79.31%,90.91%,差别(P=0.024)具有统计学意义,与患者病理组织类型、性别及年龄无关。在胶质瘤中Smac的表达明显与XIAP的表达呈负相关(rs=-0.272,P=0.036)。结论1、Smac的表达与胶质瘤恶性程度呈负相关,胶质瘤的发生、发展可能与其在恶性经胶质瘤的表达减少和缺失有关； 2、XIAP的表达与胶质瘤恶性程度呈正相关,可能与胶质瘤细胞的恶性增殖密切相关； 3、人脑胶质瘤中Smac的表达与XIAP的表达呈负相关,二者相互制约； 4、Smac和XIAP的进一步深入研究也许会发现胶质瘤化学治疗的新思路；
[Abstract]:Objective Glioma has the highest proportion compared with other intracranial tumors and can not be resected effectively after surgical intervention, which leads to recurrence easily and poor prognosis. Glioma has a high incidence and high recurrence rate. The characteristics of high mortality and low cure rate are three high and one low. The aim of this study was to investigate the expression of the second mitochondrial activator of cysteine protease (the second mitochodria-derived activator of caspase, SMAC) and X-linked inhibitor of apoptosis protein inhibitor (XIAP) in gliomas by immunohistochemistry. By analyzing the relationship between Smaca XIAP and glioma malignancy degree, it is helpful to predict the malignant degree and prognosis of glioma accurately, and to consider the occurrence of glioma from the angle of apoptosis. Methods from June 2011 to December 2013, 62 cases of gliomas were surgically resected from the second Hospital of Lanzhou University. Adopt standard, according to WHO (2007) classification and classification standard. Immunohistochemistry was used to detect streptomycin anti-biological peroxidase (SP) method. In blank control, phosphoric acid buffer was used instead of the first antibody Smac and XIAP protein to express brown granules in the cytoplasm. According to the standard immunoreactive integration method, the staining depth of positive cells was observed and the number of positive cells in the field of optical microscope was counted, and the percentage of positive cells in the total number of cells was calculated. (percentage of positive cells: no positive cells were found in 0 points (1), the number of positive cells in the total number of cells was less than 10%, 1 minute, 1125%, 2 minutes, 2550%, 3 minutes, 50%, 3 minutes, 50%, 4 points, according to the degree of popular staining (no staining was 0 points, Light yellow is 1, brown is 2, brown is 3). The immunohistochemical staining score (immunohistochemical score = percentage of positive cells PP 脳 staining intensity SI) was composed of 13 grades of 0-12, no expression (1) was 0, weak positive () was 1 to 4, positive expression () was 5 to 8. The strong positive expression was 9 to 12 points. According to the experimental data, SPSS 18.0 was used to analyze the relationship between the expression and clinical data. Results the positive expression rates of SMAC protein were 89.66 and 78.79 in low-grade gliomas and high-grade gliomas, respectively. The difference was statistically significant (P0. 000). The positive expression rates of XIAP protein in low grade glioma and high grade glioma were 79.31 and 90.91respectively. The difference (P0. 024) was statistically significant, but not related to the type of pathological tissue, sex and age. The expression of SMAC in gliomas was negatively correlated with the expression of XIAP (rs-0.272). Conclusion 1 the expression of Smac is negatively correlated with the degree of malignancy of gliomas, the occurrence and development of gliomas may be related to the decrease and deletion of SMAC expression in malignant gliomas, and the expression of XIAP in gliomas is positively correlated with the degree of malignancy of gliomas. (3) the expression of SMAC and XIAP in human glioma is negatively correlated with the expression of XIAP, and they restrict each other. 4the further study of Smac and XIAP may find a new way of chemotherapy for glioma.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

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