甘草甜素对癫痫持续状态大鼠神经保护作用及机制研究

发布时间：2018-06-24 21:25

本文选题：癫痫持续状态 + 甘草甜素　；参考：《中风与神经疾病杂志》2017年10期

【摘要】：目的研究甘草甜素(glycyrrhizin,GL)对癫痫持续状态大鼠(status epilepticus,SE)神经损伤保护作用及相关分子机制。方法取清洁健康成年雄性SD大鼠60只,采用腹腔注射氯化锂-匹罗卡品的方法建立大鼠癫痫持续状态(SE)模型,随机分为甘草甜素组(SE+GL)和生理盐水组(SE+S),分别经尾静脉给予甘草甜素及生理盐水进行干预,取正常大鼠作为对照组(control group,C);根据给予GL剂量不同将其分为高剂量组(HGL,20 mg/kg)、中剂量组(MGL,10 mg/kg)及低剂量组(LGL,5 mg/kg);通过尼氏染色(NS)观察各组大鼠SE后24 h时大脑皮质神经元损伤情况,分别采用酶联免疫吸附法(ELISA)和蛋白印迹实验(Western blot)检测各组大鼠SE后24 h时血清及大脑皮质中高迁移率族蛋白(HMGB1)的表达水平。结果 SE+S组大鼠大脑皮质神经元在癫痫持续状态后24 h明显受损,SE+GL各组皮质受损神经元数量较SE+S组减少,且在不同给药剂量组间存在显著差异(P0.05);SE+GL各组在SE后24 h时大脑皮质及血清中HMGB1的表达水平较SE+S组显著降低,差异具有统计学意义(P0.05)。结论经尾静脉给予GL能有效地减少SE后大鼠大脑皮质神经元损伤,且其神经保护作用呈剂量依赖性,其机制可能与其抑制血清及大脑皮质炎性因子HMGB1的表达有关。
[Abstract]:Objective to study the protective effect of glycyrrhizin GL on nerve injury in status epileptic state rats (status) and its molecular mechanism. Methods Epilepsy status (SE) models were established in 60 healthy male SD rats by intraperitoneal injection of lithium-pilocarpine. They were randomly divided into glycyrrhizin group (SE GL) and saline group (SE S). Normal rats were taken as control group (control group C) and divided into high dose group (20 mg/kg), middle dose group (10 mg/kg) and low dose group (LGL5 mg/kg) according to different doses of GL. Enzyme linked immunosorbent assay (Elisa) and Western blot assay (blot) were used to detect the expression of high mobility group protein (HMGB1) in serum and cerebral cortex 24 h after SE. Results the number of cortical neurons in SE S group was significantly decreased compared with that in SE S group. The expression of HMGB1 in cerebral cortex and serum in SE GL group was significantly lower than that in SE S group at 24 h after SE (P0.05). Conclusion GL via caudal vein can effectively reduce the injury of cerebral cortical neurons in SE rats, and its neuroprotective effect is dose-dependent. The mechanism may be related to the inhibition of the expression of HMGB1 in serum and cerebral cortex inflammatory factor.
【作者单位】：宁夏医科大学临床医学院;西安医学院第一附属医院神经内科;西安市中心医院神经内科;
【基金】：陕西省科技统筹创新工程计划(No.S2015TLSF0010) 陕西省教育厅基金(No.11JK0715) 陕西省卫生厅科研基金(No.2012D43)
【分类号】：R742.1

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