Hemin在大鼠局灶性脑缺血缺氧下对外源性Ngb的诱导作用的研究

发布时间：2018-06-27 16:47

本文选题：氯化血红素 + 神经球蛋白　；参考：《重庆医科大学》2014年硕士论文

【摘要】：目的：采用统计学分析方法，探讨大鼠局灶性脑缺血缺氧条件下，不同组别，氯化血红素(Hemin）对外源性神经球蛋白（Ngb）的诱导表达作用，从而进一步确定其对大鼠局灶性脑缺血缺氧的保护作用，，为临床研究提供实验依据。方法：将125只SD大鼠随机分为5组（N=25），分别为假手术对照组，手术组，Hemin处理组，外源性Ngb干预组，Hemin和外源性Ngb的联合处理组。5组大鼠分别行MCAO手术，并于术后24h分别通过免疫组化技术、RT-PCR技术、Western blot技术了解大鼠脑内Ngb的表达情况，同时记进行检测不同组别脑含水量及脑梗死体积。所有数据通过SPSS13.0软件进行统计学分析。结果：Hemin与外源性Ngb的联合处理组比较，免疫组化显示神经球蛋白阳性神经元数量较Hemin或外源性Ngb单独处理组增多；RT-PCR结果分析显示神经球蛋白在mRNA水平，Hemin及外源性Ngb单独处理组较Hemin或外源性Ngb单独处理组增多；Western blot定量结果分析显示也是这样的趋势；脑含水量分析示Hemin及外源性Ngb单独处理组较Hemin或外源性Ngb单独处理组脑水肿程度减轻；并且Hemin和外源性Ngb的联合处理组脑含水量及脑梗死体积较Hemin或外源性Ngb单独处理组有显著差异（p0.05）。结论：在大鼠局灶性脑缺血缺氧条件下，Hemin能够诱导外源性Ngb表达，并起到一定的神经保护作用。
[Abstract]:Objective: to investigate the effect of hemin on the expression of exogenous neuroglobulin (NGB) in different groups of rats with focal cerebral ischemia and hypoxia by means of statistical analysis. Thus, the protective effect on focal cerebral ischemia and hypoxia in rats was further determined, and the experimental basis was provided for clinical study. Methods: 125 Sprague-Dawley rats were randomly divided into 5 groups: sham operation control group, operation group treated with Hemin, exogenous NGB intervention group and exogenous Ngb combined treatment group, which were treated with MCAO respectively. At 24 hours after operation, the expression of NGB in rat brain was detected by RT-PCR and Western blot respectively, and the water content and infarct volume of different groups of brain were measured at the same time. All data were analyzed statistically by SPSS 13.0 software. Results compared with the combined treatment group with exogenous NGB, the number of neuroglobulin positive neurons was increased by immunohistochemistry compared with that of Hemin or exogenous NGB alone. The results of RT-PCR showed that the amount of neuroglobulin in the treated group was more than that in the Hemin or exogenous NGB alone treatment group, and the quantitative analysis showed the same trend. Brain water content analysis showed that the brain edema degree of Hemin and exogenous NGB alone treatment group was lower than that of Hemin or exogenous NGB alone treatment group. The cerebral water content and cerebral infarction volume in the combined treatment group of Hemin and exogenous NGB were significantly different from those in the Hemin or exogenous NGB alone group (p0.05). Conclusion: Hemin can induce exogenous NGB expression in rats with focal cerebral ischemia and hypoxia and play a neuroprotective role.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743

【共引文献】