SELDI蛋白芯片检测技术在脑膜炎分型中的应用研究

发布时间：2018-06-28 21:19

  本文选题：脑膜炎 + SELDI-TOF-MS　； 参考：《吉林大学》2016年硕士论文

【摘要】：脑膜炎在颅内感染疾病中属常见病、多发病,以化脓性脑膜炎、结核性脑膜炎及病毒性脑膜炎为主,共同的临床表现有头痛、发热、呕吐甚至惊厥、昏迷等。脑膜炎患者若得不到及时有效的治疗,将会遗留严重后遗症甚至死亡。脑膜炎的早期诊断主要依靠临床表现、影像学检查及实验室检验。目前脑脊液中直接检出致病微生物仍是临床诊断的“金标准”,然而由于传统病原学检测方法阳性率低、耗时较长,对脑膜炎类型的早期诊断不具备明显提示意义,因此急需建立一种高效快速并且敏感特异的诊断方法。目的:应用表面增强激光解吸电离飞行时间质谱(surface-enhanced laser desorption ionization time-of-flight mass spectrometry,SELDI-TOF-MS)联合蛋白芯片技术建立三种类型脑膜炎(化脓性脑膜炎、结核性脑膜炎、病毒性脑膜炎)分型诊断模型。方法:采集62例脑膜炎患者(化脓性脑膜炎16例、结核性脑膜炎18例、病毒性脑膜炎28例)脑脊液样本,高速离心后-80℃冰箱保存,样本在一个冻融周期内同批进行分析。采用疏水芯片H50和SELDI-TOF-MS技术检测62例脑脊液样本,应用Biomarker Wizard软件分析找出差异蛋白峰,用SPSS 16.0软件对数据进行统计学分析,应用Biomarker Patterns 5.0软件建立脑膜炎分型诊断模型。结果:共采集到蛋白峰129个,经软件分析有统计学差异的蛋白峰共40个,利用m/z=2176.205、m/z=2326.05、m/z=2436.154、m/z=3399.328、m/z=4125.172、m/z=8927.876、m/z=14675.06七个差异表达的蛋白质峰成功建立脑膜炎分类决策树,准确度为93.548%(58/62)。通过自身盲法验证模型对脑膜炎分型的准确度为93.333%(28/30)。结论:由7个差异蛋白峰构成的分类决策树为脑膜炎的分型提供了新的借鉴和参考。
[Abstract]:Meningitis is a common disease in intracranial infection, mainly suppurative meningitis, tuberculous meningitis and viral meningitis. The common clinical manifestations are headache, fever, vomiting and even convulsion, coma and so on. Meningitis patients without timely and effective treatment will leave serious sequelae or even death. The early diagnosis of meningitis depends mainly on clinical manifestations, imaging and laboratory tests. At present, direct detection of pathogenic microorganisms in cerebrospinal fluid (CSF) is still the "gold standard" for clinical diagnosis. However, due to the low positive rate of traditional etiological methods and long time consuming, it has no obvious significance for the early diagnosis of meningitis type. Therefore, it is urgent to establish an efficient, rapid and sensitive diagnostic method. Objective: to establish three types of meningitis (suppurative meningitis, tuberculous meningitis, viral meningitis) by surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) and protein chip technique. Methods: cerebrospinal fluid (CSF) samples were collected from 62 cases of meningitis (16 cases of suppurative meningitis, 18 cases of tuberculous meningitis and 28 cases of viral meningitis). The samples were stored in refrigerator at -80 鈩，

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