脉冲射频对神经源性疼痛大鼠脊髓GFAP、PICK1水平的影响

发布时间：2018-07-02 12:26

本文选题：脉冲射频 + 神经源性疼痛　；参考：《中国老年学杂志》2017年12期

【摘要】：目的探讨脉冲射频对神经源性疼痛(NP)大鼠脊髓胶质原纤维酸性蛋白(GFAP)、蛋白激酶C-α1相互作用蛋白(PICK1)水平的影响。方法 150只雄性成年SD大鼠随机分为假性手术组、模型组、脉冲射频2 Hz组、4 Hz组、8 Hz组,每组30只。分别于术前、术后1、3、7、14 d记录各组大鼠热缩腿反射潜伏期(TWL)、机械缩足反射阈值(MWT),抽取各组大鼠脊髓应用Western印迹法测定各组脊髓中GFAP蛋白、PICK1蛋白水平。结果模型组、2 Hz组、4 Hz组、8 Hz组术后1、3、7、14 d TWL、MWT水平显著短于假性手术组(P0.05),模型组显著短于射频2 Hz组、4 Hz组、8 Hz组(P0.05);随着射频频率增加,TWL、MWT水平显著升高(P0.05),模型组、射频2 Hz组、4 Hz组、8 Hz组脊髓术后1、3、7、14 d GFAP、PICK1表达水平显著高于假性手术组(P0.05),模型组高于射频2 Hz组、4 Hz组、8 Hz组(P0.05);而射频组中随着作用频率的增加,脊髓GFAP蛋白、PICK1蛋白表达水平显著下降(P0.05)。结论脉冲射频能有效减轻坐骨神经压迫性损伤所致NP大鼠热和机械性痛觉过敏,能有效抑制脊髓GFAP、PICK1表达;随着射频频率的增加,效果越理想,射频对NP有治疗效果。
[Abstract]:Objective to investigate the effects of pulsed radio frequency on the levels of glial fibrillary acidic protein (GFAP) and protein kinase C- 伪 1 interacting protein (PICK1) in spinal cord of rats with neurogenic pain (NP). Methods 150 adult male Sprague-Dawley rats were randomly divided into sham operation group (n = 30), model group (n = 30) and pulse radiofrequency frequency (RF) group (n = 30) (n = 30). The latent period of thermal contraction leg reflex (TWL) and mechanical foot reflex threshold (MWT) were recorded before and 1 day after operation. The levels of GFAP protein and PICK1 protein in spinal cord of each group were measured by Western blotting. Results the level of TWL MWT in the model group was significantly shorter than that in the sham operation group (P0.05), the MWT level in the model group was significantly shorter than that in the 4 Hz group (P0.05), the MWT level in the model group was significantly higher with the increase of radio frequency (P0.05), the MWT level in the model group was significantly lower than that in the model group (P0.05), and the MWT level in the model group was significantly lower than that in the 4 Hz group (P0.05). The expression of GFAPPICK1 was significantly higher in the 2 Hz / 4 Hz group than in the sham operation group (P0.05), and in the model group was higher than that in the 2 Hz / 4 Hz group (P0.05), while in the radiofrequency group with the increase of the frequency of action, the expression of PICK1 was significantly higher than that of the control group (P0.05), and the expression of PICK1 was significantly higher in the model group than in the 2 Hz / 4 Hz group (P0.05). The expression of GFAP protein PICK1 in spinal cord decreased significantly (P0.05). Conclusion Pulsed radiofrequency can effectively reduce heat and mechanical hyperalgesia induced by compression injury of sciatic nerve in NP rats, and can effectively inhibit the expression of PICK1 in spinal cord GFAPP. With the increase of RF frequency, the better the effect is, the better the therapeutic effect of RF is on NP.
【作者单位】：新乡市中心医院疼痛科;
【基金】：河南省科技厅科技发展计划(14A320075)
【分类号】：R741

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