特制牛肉汤对脑缺血大鼠外周血白细胞介素-1β和干扰素-γ的影响

发布时间：2018-07-02 12:33

本文选题：特制牛肉汤 + 脑缺血　；参考：《中国老年学杂志》2017年22期

【摘要】：目的研究特制牛肉汤对脑缺血大鼠外周血白细胞介素(IL)-1β及干扰素(IFN)-γ的影响。方法将96只雄性SD大鼠按随机数字表分为假手术组、模型组、特制牛肉汤组和肌肽组,每组24只。模型组、特制牛肉汤组和肌肽组采用线栓法制备永久性大脑中动脉闭塞模型(p MCAO),于术后清醒2 h内首次灌胃给药,特制牛肉汤组予600 mg·kg~(-1)·d~(-1)特制牛肉汤,肌肽组予600 mg·kg~(-1)·d~(-1)肌肽水溶液。假手术组与模型组予等量无菌生理盐水,每日2次,各组大鼠分别于造模后1 d,3 d和7 d处死,各组各时点8只大鼠。采用Longa评分法进行神经功能缺损评分,2,3,5-氯化三苯基四氮唑(TTC)染色法测定脑梗死体积分数、酶联免疫吸附法(ELISA)测外周血中IL-1β、IFN-γ表达。结果脑缺血后,各时点模型组大鼠神经功能评分显著上升;与模型组相比,予特制牛肉汤7 d后,脑缺血大鼠神经功能评分显著下降(P0.05)。脑缺血后,模型组、特制牛肉汤组和肌肽组均可见大脑中动脉供血区梗死灶。与模型组相比,予特制牛肉汤和肌肽1 d和3 d后,脑梗死体积分数有下降趋势,但无统计学差异(P0.05);予特制牛肉汤7 d后,脑梗死体积分数显著降低(P0.01)。脑缺血后,各时点模型组大鼠外周血IL-1β水平显著上升(P0.05),与模型组相比,予特制牛肉汤7 d后,IL-1β水平明显下降(P0.05);予肌肽7 d后,IL-1β有下降趋势,但无统计学差异(P0.05)。脑缺血后3 d和7 d,模型组大鼠外周血IFN-γ水平显著上升(P0.05),与模型组相比,予特制牛肉汤和肌肽7 d后,IFN-γ水平明显下降(P0.01)。结论特制牛肉汤通过下调促炎细胞因子IL-1β、IFN-γ表达,从而减轻脑缺血大鼠炎症反应,改善神经功能缺损症状,对脑起保护作用。
[Abstract]:Objective to study the effects of special beef decoction on interleukin (IL)-1 尾 and interferon (IFN)-纬 in peripheral blood of rats with cerebral ischemia. Methods 96 male Sprague-Dawley rats were randomly divided into sham-operation group, model group, special beef soup group and carnosine group with 24 rats in each group. Model group, special beef decoction group and carnosine group were used to establish permanent middle cerebral artery occlusion model (pMCAO) by thread occlusion. The rats in the special beef decoction group were given 600 mg kg ~ (-1) d ~ (-1) special beef soup, and the carnosine group were given 600 mg kg ~ (-1) d ~ (-1) carnosine solution. The sham-operation group and the model group were given the same amount of aseptic saline twice a day. The rats in each group were killed on the 1st day and 7th day, respectively. There were 8 rats in each group at each time point. The neurological function defect score (NDS) was measured by Longa score method. The volume fraction of cerebral infarction was measured by TTC staining and the expression of IL-1 尾 -IFN- 纬 in peripheral blood was measured by enzyme linked immunosorbent assay (Elisa). Results after cerebral ischemia, the neurological function score of the model group increased significantly, compared with the model group, after 7 days of special beef decoction, the neurological function score of the cerebral ischemic rats decreased significantly (P0.05). After cerebral ischemia, the infarcts of the middle cerebral artery were found in the model group, the special beef decoction group and the carnosine group. Compared with the model group, the volume fraction of cerebral infarction decreased after 1 and 3 days of treatment with special beef soup and carnosine (P0.05), and the volume fraction of cerebral infarction decreased significantly after 7 days (P0.01). After cerebral ischemia, the level of IL-1 尾 in peripheral blood of the model group increased significantly (P0.05), compared with the model group, the level of IL-1 尾 decreased significantly after 7 days of treatment with special beef decoction (P0.05), and the level of IL-1 尾 decreased after 7 days of treatment with carnosine, but there was no statistical difference (P0.05). The level of IFN- 纬 in peripheral blood of the model group increased significantly 3 days and 7 days after cerebral ischemia (P0.05). Compared with the model group, the level of IFN- 纬 in the model group decreased significantly after 7 days of treatment with special beef decoction and carnosine (P0.01). Conclusion the special beef decoction can reduce the inflammatory reaction, improve the symptom of nerve function defect and protect the brain by down-regulating the expression of IL-1 尾 -IFN- 纬 in rats with cerebral ischemia.
【作者单位】：南京中医药大学;南京医科大学康达学院;
【基金】：江苏省优势学科项目资助项目(No.YSHL0201-26) 江苏省青蓝工程项目资助
【分类号】：R743.3

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