基底节脑出血对发育期大鼠行为发育及脑区多巴胺转运体的影响

发布时间：2018-07-06 11:04

本文选题：脑出血 + 发育期　；参考：《福建医科大学》2014年硕士论文

【摘要】：目的：探讨发育期大鼠基底节出血对大鼠行为发育及脑内多巴胺转运体（DAT）表达的影响。方法： 1.新生同窝SD大鼠（P10）随机分为脑出血（CH）组、假手术（SH）组和正常对照（NC）组，每组各16只。 2.利用立体定位仪向SD大鼠脑尾状核注入自体血，建立脑出血模型，SH组只刺入而不注血，NC组不做任何处理。 3.分别于术后第1天（即P11）、术后第3天（即P13）、术后第7天（即P17）、术后第14d天即P24）采用Berderson评分法进行大鼠神经功能评分。 4.术后第14天（即P24)通过开场实验及Lat迷宫进行大鼠行为学检测，连续测试3天。 5.术后第17天（即P27)采用H.E染色观察大鼠脑出血后病理变化，免疫组织化学染色测定DAT的表达。结果： 1.CH组大鼠开场实验中的穿越格子数、直立次数均较SH组及NC组大鼠明显增多，差异有统计学意义(P 0．05)； CH组大鼠在Lat迷宫内穿越角落数、直立次数、理毛次数均较SH组及NC组大鼠显著增加,差异有统计学意义(P 0．05)。 2. H.E染色显示CH组血肿处可见一小空腔形成，可见少许淋巴细胞浸润及少许胶质瘢痕形成；SH组未见明显血肿，亦无空腔形成；NC组无炎性细胞浸润，细胞分布正常。 3. CH组大鼠脑内DAT免疫反应阳性产物较NC组及SH组显著增多，差异具有统计学意义(P 0．05)；而SH组与NC组比较差异无统计学意义(PO.O5)。 4.基底节区DAT蛋白与大鼠开场实验中穿越格子数、直立次数、理毛次数及Lat迷宫中穿越角落数、直立次数、理毛次数成正相关，具有统计学意义（P 0.05）。结论： 1.发育期大鼠基底节局灶性出血，可修复性、代偿性强，，多不遗留严重神经运动功能障碍。 2.脑基底节出血所致的神经损伤可导致发育期大鼠出现自主活动增多、非选择性注意水平降低等多动、注意缺陷等远期行为异常。 3.脑内多巴胺转运体（DAT）表达水平的增高可能与发育期大鼠脑基底节损伤后多动、注意缺陷等发育行为异常有关。
[Abstract]:Aim: to investigate the effects of basal ganglia hemorrhage on behavioral development and dopamine transporter (DAT) expression in rat brain. Methods: 1. Neonatal Sprague-Dawley rats (P10) were randomly divided into three groups: intracerebral hemorrhage (Ch) group, sham operation (SH) group and normal control (NC) group, with 16 rats in each group. Autologous blood was injected into the caudate nucleus of the brain of SD rats by stereotactic locator. On the 1st day (P11), the 3rd day (P13), the 7th day (P17) and the 14th day after operation (P24), the neurological function score of rats was evaluated by Berderson's scoring method. On the 14th day after operation (P24), the behavior of rats was measured by open field experiment and Lat maze. The rats were tested continuously for 3 days. On the 17th day after operation (P27), the expression of DAT was determined by immunohistochemical staining, and the pathological changes of rats with intracerebral hemorrhage (ICH) were observed by H. E staining. Results: 1. Compared with SH group and NC group, the number of grid crossing and the number of upright in Ch group were significantly higher than those in SH group and NC group (P 0.05), the rats in Ch group passed through corner number in Lat labyrinth, and the number of upright rats in Ch group was significantly higher than that in SH group and NC group (P < 0.05). Compared with SH group and NC group, the times of shaving were significantly higher than that of SH group and NC group, the difference was statistically significant (P 0.05). E staining showed that there was a small cavity formation in hematoma, a little lymphocyte infiltration and a little glial scar formation in Ch group. No hematoma was found in SH group, and no inflammatory cell infiltration was found in NC group, and the cell distribution was normal in NC group. The expression of DAT immunoreactive products in brain of Ch group was significantly higher than that of NC group and SH group (P 0.05), but there was no significant difference between SH group and NC group (PO. O 5). 4. DAT protein in basal ganglia area was positively correlated with the number of traverses, the number of upright positions, the number of haircuts and the number of crossing corners in Lat labyrinth in the opening experiment of rats (P 0.05). Conclusion: 1. The focal hemorrhage of basal ganglia in developing rats is repairable and compensatory, and it does not leave behind serious neuromotor dysfunction. 2. The nerve injury caused by basal ganglia hemorrhage may lead to the increase of spontaneous activity, the decrease of non-selective attention level, the abnormal long-term behavior of attention deficit and so on. 3. The increased expression of dopamine transporter (DAT) in brain may be related to abnormal developmental behavior such as hyperactivity and attention deficit after basal ganglion injury in developing rats.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.34

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