人颞叶内侧癫痫海马组织星形胶质细胞Syntrophin的表达变化

发布时间：2018-07-14 22:37

【摘要】：目的观察人颞叶内侧癫痫(MTLE)海马组织星形胶质细胞syntrophin的表达变化。方法 2015年4月至2016年7月,17例癫痫患者手术切除的海马组织,依据苏木素-伊红(HE)染色及胶质原纤维酸性蛋白、神经元核抗原免疫组织化学染色结果,分为MTLE组13例和非MTLE组4例。免疫荧光双重组织化学法及免疫荧光组织化学法观察syntrophin的表达。结果 MTLE组星形胶质细胞大量增生,神经元明显减少。syntrophin在星形胶质细胞膜及足突处表达。与非MTLE组相比,MTLE组syntrophin在血管周围星形胶质细胞足突处分布减少,在其他部位分布增加。图像分析显示,与非MTLE组相比,MTLE组syntrophin蛋白量显著增多(t=5.421,P0.001)。结论海马组织syntrophin在星形胶质细胞上的分布及其整体表达变化可能与MTLE发生相关。
[Abstract]:Objective to observe the expression of syntrophin in hippocampal astrocytes of human medial temporal lobe epilepsy (MTLE). Methods from April 2015 to July 2016, the hippocampal tissues of 17 epileptic patients were surgically resected. The results of HE staining, glial fibrillary acidic protein (GFAP) and neuronal nuclear antigen immunohistochemical staining were used. The patients were divided into MTLE group (n = 13) and non-MTLE group (n = 4). The expression of syntrophin was observed by immunofluorescence double histochemistry and immunofluorescence histochemistry. Results in MTLE group, astrocytes proliferated and neurons decreased the expression of .syntrophin in astrocyte membrane and foot process. Compared with the non-MTLE group, the distribution of syntrophin in the perivascular astrocyte podocytes decreased, but increased in other parts. Image analysis showed that the amount of syntrophin protein in the MTLE group was significantly higher than that in the non-MTLE group (t = 5.421 / P0.001). Conclusion the distribution of syntrophin in astrocytes and its global expression in hippocampus may be related to the occurrence of MTLE.
【作者单位】：首都医科大学北京市神经外科研究所病理生理室首都医科大学附属北京天坛医院;首都医科大学附属北京天坛医院神经外科;北德克萨斯大学医学中心神经与药理系;
【基金】：北京市自然科学基金项目(No.7152027)
【分类号】：R742.1

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