星型胶质细胞在帕金森病大鼠中脑、脑室下区的分布和体外培养分化特征

发布时间：2018-07-14 22:44

【摘要】：目的:观察星形胶质细胞在帕金森病(Parkinson's disease,PD)模型大鼠脑室下区、中脑的分布并进行PD模型大鼠脑室下区星形胶质细胞的体外培养、纯化和鉴定。方法:采用免疫荧光方法观察胶原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)在PD模型大鼠中脑、脑室下区的表达;取PD模型大鼠侧脑室下区细胞进行体外培养、传代、贴壁分化和免疫荧光法鉴定。结果:(1)GFAP阳性细胞在PD模型大鼠中脑病侧较健侧明显增生,且GFAP荧光定量强度值在病侧较健侧明显增大(P0.05);GFAP阳性细胞在侧脑室、第三脑室和第四脑室室周区、视上核、下丘脑室旁核外侧大细胞部、正中隆起大量分布;(2)PD模型大鼠侧脑室下区培养分化的细胞具有星形胶质细胞的典型形态,其中原浆性星形胶质细胞突起粗短,分支多;纤维性星形胶质细胞突起细长,分支较少。结论:(1)星形胶质细胞在PD模型大鼠中脑和脑室下区的分布有明显区域性,可能与病侧的病理性增生相关,与相应区域的生理活动、调节功能及有关;(2)取PD模型大鼠侧脑室下区进行体外培养、分化获得星形胶质细胞的实验方法可靠。
[Abstract]:Aim: to observe the distribution of astrocytes in the subventricular and midbrain of Parkinson's disease (PD) model rats and to culture, purify and identify the astrocytes in the subventricular area of PD model rats in vitro. Methods: the expression of collagen fibrillary acidic protein (glial fibrillary acidic) in the midbrain and subventricular region of PD model rats was observed by immunofluorescence method, and the cells in the lateral subventricular area of PD model rats were cultured in vitro. Adherent differentiation and immunofluorescence identification. Results: (1) GFAP positive cells proliferated significantly in the middle encephalopathy side of PD model rats compared with the healthy side, and the fluorescence quantitative intensity of GFAP increased significantly in the diseased side than in the healthy side (P0.05) the GFAP positive cells were located in the lateral ventricle, the third ventricle and the periventricular area of the fourth ventricle, and the supraoptic nucleus. (2) the differentiated cells in the subventricular region of the lateral ventricle of PD model rats had typical astrocyte morphology, in which the proplasmic astrocytes had short protrusions and many branches in the lateral paraventricular nucleus of hypothalamus, and a large number of the cells were located in the lateral paraventricular nucleus of hypothalamus. Fibrous astrocytes have slender processes and few branches. Conclusion: (1) the distribution of astrocytes in the midbrain and subventricular regions of PD rats is obviously regional, which may be related to the pathological hyperplasia of the diseased side and the physiological activities of the corresponding regions. (2) the subventricular area of PD model rats were cultured in vitro and the astrocytes were obtained by differentiation.
【作者单位】：泰州市第四人民医院泰州市脑科医院神经内科;东南大学医学院人体结构与神经科学学系;
【基金】：东南大学国家自然科学基金预研项目(XJ0623250,9223001405)
【分类号】：R742.5

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