人脐血间充质干细胞移植对脑梗死兔神经功能障碍和头部核磁共振波谱的影响

发布时间：2018-07-20 18:47

【摘要】：目的观察人脐血间充质干细胞移植对缺血再灌注兔神经功能障碍和头部核磁共振波谱参数的影响。方法采集足月新生儿脐带血60~80 ml,分离h UCB-MSCs,并体外培养。将造模成功的大脑中动脉栓塞模型30只兔随机分为脑梗死组、干细胞组、生理盐水组,每组10只,后两组移植人脐血间充质干细胞和生理盐水。移植48 h、2 w时,采用Purdy评分进行神经损害严重程度评估,行核磁共振及氢质子核磁共振波谱检查,进行梗死体积、及梗死中心区N-乙酰天门冬氨酸、胆碱和肌酸等代谢物含量的测定。结果与脑梗死组及生理盐水组相比,干细胞组NSS评分较低,差异具有统计学意义(P0.05);生理盐水组与脑梗死组NSS评分无明显差异(P0.05)。与脑梗死组和生理盐水组相比,干细胞组梗死体积明显减小(P0.05)。与假手术组相比,干细胞组、脑梗死组与生理盐水组NAA/Cr、Cho/Cr比值均明显下降,Lac/Cr比值均显著上升(P0.01);但干细胞组NAA/Cr、Cho/Cr比值显著高于脑梗死组及生理盐水组(P0.05),Lac/Cr比值显著低于后两组(P0.05);而生理盐水组NAA/Cr、Cho/Cr、Lac/Cr比值与脑梗死组之间无明显差异(P0.05)。结论脐血间充质干细胞移植能提高脑梗死兔脑NAA/Cr、Cho/Cr比值,降低Lac/Cr比值,缩小梗死范围,明显改善兔神经功能。
[Abstract]:Objective to investigate the effects of transplantation of human umbilical cord blood mesenchymal stem cells (hCBMSCs) on neurological dysfunction and head nuclear magnetic resonance spectroscopy (NMR) in rabbits with ischemia reperfusion. Methods umbilical cord blood samples from full-term neonates were collected from 6080 ml and hUCB-MSCs were isolated and cultured in vitro. Thirty rabbits with successful middle cerebral artery embolization were randomly divided into three groups: cerebral infarction group, stem cell group, physiological saline group, 10 rabbits in each group, the latter two groups were transplanted with human umbilical cord blood mesenchymal stem cells and normal saline. Purdy score was used to evaluate the severity of nerve damage, nuclear magnetic resonance (MRI) and proton nuclear magnetic resonance spectroscopy (HNMR) were used to measure the infarct volume and N- acetyl aspartate in the center of the infarct. Determination of metabolites such as choline and creatine. Results the NSS score of stem cell group was lower than that of cerebral infarction group and normal saline group (P0.05), but there was no significant difference between normal saline group and cerebral infarction group (P0.05). Compared with cerebral infarction group and normal saline group, the infarct volume of stem cell group decreased significantly (P0.05). Compared with the sham operation group, the stem cell group, In cerebral infarction group and saline group, the ratio of NAA / CrCho-Cr decreased significantly (P0.01), but the ratio of NAA- / CrCho-Cho-Cr in stem cell group was significantly higher than that in cerebral infarction group and saline group (P0.05), but the ratio of NAA- CrChoCrChoCr-Lacr / Cr in normal saline group was significantly lower than that in the latter two groups (P0.05), but the ratio of NAA- CrChoCrChoCrChoCr-Lacr / Cr in stem cell group was significantly higher than that in cerebral infarction group and saline group (P0.05). There was no significant difference between cerebral infarction group (P0.05). Conclusion umbilical cord blood mesenchymal stem cell transplantation can improve the ratio of NAA / Cr / Cr, decrease the ratio of Lacr / Cr, reduce the size of infarct and improve the neurological function of rabbits with cerebral infarction.
【作者单位】：解放军第一0五医院神经内科;
【基金】：国家自然科学基金(No.81171108)
【分类号】：R743.3

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