腺苷A2A受体对新生小鼠离体延髓脑片基本节律性呼吸放电的调节作用
发布时间：2018-08-10 19:38
【摘要】：目的:探讨腺苷A2A受体对新生小鼠离体延髓脑片基本节律性呼吸放电(RRDA)的调节作用。方法:制备新生小鼠离体延髓脑片,分别使用含0.0、2.5、5.0、10.0、20.0 nmol/L腺苷A2A受体激动剂CGS21680或拮抗剂SCH58261的人工脑脊液(ACSF)灌流脑片,每个浓度灌流10 min,在每个浓度灌流第6 min时,使用吸附电极记录脑片RRDA,分析吸气时程(TI)、放电积分幅度(IA)和呼吸周期(RC)。结果:CGS21680可以缩短RC,增加TI和IA(P0.001),对RRDA有兴奋作用。SCH58261则可以延长RC,减小TI和IA(P0.001),对RRDA有抑制作用。结论:腺苷A2A受体参与调节新生小鼠延髓RRDA,激活腺苷A2A受体对RRDA有兴奋作用。
[Abstract]:Aim: to investigate the regulatory effect of adenosine A 2A receptor on the basic rhythmic respiratory discharge (RRDA) in isolated medulla oblongata of newborn mice. Methods: in vitro medulla oblongata slices of newborn mice were prepared and perfused with the artificial cerebrospinal fluid (ACSF) containing 0.02.55.010.0 nmol/L A2A receptor agonist CGS21680 or antagonist SCH58261 for 10 minutes, at 6 min in each concentration. The absorption electrode was used to record the RRDAs of brain slices. The integral amplitude (IA) of inspiratory (TI), discharge and the (RC). Of respiratory cycle were analyzed. Results: CGS21680 could shorten RCand increase TI and IA (P0.001), but it could prolong RRDA, decrease TI and IA (P0.001) and inhibit RRDA. Conclusion: adenosine A 2A receptor is involved in the regulation of medulla oblongata of newborn mice, and the activation of adenosine A 2A receptor can stimulate RRDA.
【作者单位】： 新乡医学院机能学实验室;新乡市结核病防治所;新乡医学院三全学院;
【基金】：河南省科技厅重点攻关项目102102310156 新乡市科技局重点攻关项目ZG14004
【分类号】：R741
本文编号：2175997
[Abstract]:Aim: to investigate the regulatory effect of adenosine A 2A receptor on the basic rhythmic respiratory discharge (RRDA) in isolated medulla oblongata of newborn mice. Methods: in vitro medulla oblongata slices of newborn mice were prepared and perfused with the artificial cerebrospinal fluid (ACSF) containing 0.02.55.010.0 nmol/L A2A receptor agonist CGS21680 or antagonist SCH58261 for 10 minutes, at 6 min in each concentration. The absorption electrode was used to record the RRDAs of brain slices. The integral amplitude (IA) of inspiratory (TI), discharge and the (RC). Of respiratory cycle were analyzed. Results: CGS21680 could shorten RCand increase TI and IA (P0.001), but it could prolong RRDA, decrease TI and IA (P0.001) and inhibit RRDA. Conclusion: adenosine A 2A receptor is involved in the regulation of medulla oblongata of newborn mice, and the activation of adenosine A 2A receptor can stimulate RRDA.
【作者单位】： 新乡医学院机能学实验室;新乡市结核病防治所;新乡医学院三全学院;
【基金】：河南省科技厅重点攻关项目102102310156 新乡市科技局重点攻关项目ZG14004
【分类号】：R741
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