神经干细胞移植脑缺血模型大鼠神经细胞凋亡、分化及神经行为学的变化

发布时间：2018-08-10 20:41

【摘要】：背景:近年来,神经干细胞移植已成为治疗脑缺血等脑损伤性疾病的研究热点,但机制尚未完全阐明。目的:观察神经干细胞移植治疗脑缺血大鼠对其神经细胞凋亡、分化及神经行为的影响。方法:90只脑缺血模型造模成功SD大鼠随机分为3组,其中模型组30只、磷酸盐缓冲液组(注射磷酸盐缓冲液)30只、神经干细胞组(移植神经干细胞)30只;假手术组30只仅在麻醉状态下分离暴露血管,不进行阻断血流的操作;对照组30只不进行任何操作。对比各组大鼠在造模后第3,7,14天的神经功能评分、脑梗死体积、神经细胞凋亡数、Brdu/Neu N及Brdu/GFAP的表达差异。结果与结论:(1)造模后第7,14天,神经干细胞组大鼠的神经缺损症状评分、脑梗死体积和神经细胞凋亡数均显著低于模型组和磷酸盐缓冲液组(P0.05),模型组和磷酸盐缓冲液组比较,差异无显著性意义(P0.05);(2)造模后第3,7,14天,对照组、假手术组的神经缺损症状评分和神经细胞凋亡数均显著低于模型组、磷酸盐缓冲液组和神经干细胞组大鼠(P0.05);(3)造模后第3天,模型组、磷酸盐缓冲液组和神经干细胞组的Brdu/NeuN阳性细胞计数差异无显著性意义(P0.05);造模后第7,14天,神经干细胞组的Brdu/NeuN阳性细胞计数均显著高于模型组和磷酸盐缓冲液组(P0.05),模型组和磷酸盐缓冲液组比较差异无显著性意义(P0.05);(4)造模后第3,7,14天,神经干细胞组的Brdu/GFAP阳性细胞计数均显著低于模型组和磷酸盐缓冲液组(P0.05),模型组和磷酸盐缓冲液组比较差异无显著性意义(P0.05);(5)结果表明,移植神经干细胞治疗脑缺血大鼠可以促进侧脑室下区的内源性神经干细胞向神经元方向分化,减少神经细胞凋亡,改善缺血对大鼠神经功能的损害作用。
[Abstract]:Background: in recent years, neural stem cell transplantation has become a research focus in the treatment of cerebral ischemia and other brain injury diseases, but the mechanism has not been fully clarified. Aim: to observe the effects of neural stem cell transplantation on neuronal apoptosis, differentiation and neural behavior in rats with cerebral ischemia. Methods 90 Sprague-Dawley rats were randomly divided into three groups: model group (n = 30), phosphate buffer group (n = 30) and neural stem cell group (n = 30). The sham operation group (30 rats) only separated the exposed blood vessels under anaesthesia and did not block the blood flow, while the control group (30 cases) did not perform any operation. The neurological function score, cerebral infarct volume, neuron apoptosis and the expression of Brdu-Neu N and Brdu/GFAP in rats were compared on the 3rd day and 14th day after the model. Results and conclusion: (1) the neurological deficit symptom scores, cerebral infarct volume and the number of neuronal apoptosis in the neural stem cell group were significantly lower than those in the model group and phosphate buffer group (P0.05). There was no significant difference (P0.05); (2) on the 3rd day after modeling. In the control group, the nerve defect symptom score and the number of neuronal apoptosis in the sham operation group were significantly lower than those in the model group. The rats in the phosphate buffer solution group and the neural stem cell group (P0.05); (3) were significantly lower than those in the model group on the 3rd day after modeling. The number of Brdu/NeuN positive cells in model group, phosphate buffer group and neural stem cell group had no significant difference (P0.05). The number of Brdu/NeuN positive cells in neural stem cells group was significantly higher than that in model group and phosphate buffer group (P0.05). There was no significant difference between model group and phosphate buffer group (P0.05); (4). The number of Brdu/GFAP positive cells in neural stem cells group was significantly lower than that in model group and phosphate buffer group (P0.05). There was no significant difference between model group and phosphate buffer group (P0.05); (5). Transplantation of neural stem cells in cerebral ischemic rats can promote the differentiation of endogenous neural stem cells into neurons, reduce the apoptosis of nerve cells, and improve the damage effect of ischemia on the neural function of rats.
【作者单位】：潍坊医学院附属益都中心医院神经内科;潍坊医学院附属益都中心医院内分泌科;
【分类号】：R-332;R743.3

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