重组人粒细胞集落刺激因子对缺血缺氧性脑损伤新生大鼠凋亡诱导因子表达的影响

发布时间：2018-08-12 11:50

【摘要】：目的:研究分析重组人粒细胞集落刺激因子对新生SD大鼠凋亡诱导因子表达的影响。方法:随机选取7日龄SD大鼠36只,随机分为假手术组(A组)、脑缺血再灌注组(B组)和药物治疗组(C组),每组各12只。采用Longa线栓法制作大鼠大脑动脉闭塞再灌注模型,C组大鼠在脑缺血2 h后及24 h时给予药物灌注,其余2组给予大鼠注射等量的0.9%氯化钠注射液。实验结束后,采用Longa 5分制标准评分法在24 h时对大鼠的神经功能进行评分,采用免疫组织化学法对大鼠的组织磷酸化c-jun氨基末端激酶、磷酸化细胞外信号调节蛋白激酶进行检测,同时采用原位末端转移酶标记对神经细胞凋亡、TTC染色测脑梗死体积进行相关检测。对凋亡相关基因的表达及凋亡水平进行评价。结果:A组仅有一小部分凋亡细胞,B、C 2组在灌注24 h后可以发现大量的凋亡细胞,细胞核均呈棕褐色,主要分布在缺血脑组织中周围;C组脑梗死程度较B组减轻(P0.01)。结论:重组人粒细胞集落刺激因子可以起到保护新生SD大鼠神经细胞的作用,避免发生缺血缺氧性脑损伤,这种糖蛋白与细胞凋亡相关基因的表达有关。
[Abstract]:Aim: to study the effect of recombinant human granulocyte colony stimulating factor (rhGCSF) on the expression of apoptosis-inducing factor in neonatal SD rats. Methods: Thirty-six 7-day-old SD rats were randomly divided into sham-operated group (group A), cerebral ischemia-reperfusion group (group B) and drug therapy group (group C) with 12 rats in each group. The cerebral artery occlusion reperfusion model of rats was established by Longa thread occlusion. Rats in group C were perfused with drugs at 2 hours and 24 hours after cerebral ischemia, and the other two groups were given the same amount of 0.9% sodium chloride injection. At the end of the experiment, the nerve function of the rats was evaluated by the standard Longa scoring method at 24 h, and the phosphorylated c-jun amino-terminal kinases in the tissues of the rats were assessed by immunohistochemical method. Extracellular signal regulated protein kinase (ESRK) was detected by phosphorylation and in situ terminal transferase (TTC) staining was used to detect the volume of cerebral infarction. The expression of apoptosis-related genes and the level of apoptosis were evaluated. Results A large number of apoptotic cells were found in group B _ (2) after 24 hours of perfusion, and the nuclei were brown, mainly distributed in ischemic brain tissue. The degree of cerebral infarction in group C was less than that in group B (P0.01). Conclusion: recombinant human granulocyte colony stimulating factor can protect newborn SD rat neurons from ischemic hypoxic brain injury. This glycoprotein is related to the expression of apoptosis-related genes.
【作者单位】：上海交通大学医学院附属第九人民医院儿科;
【基金】：上海市高校选拔优秀青年教师专项基金(ZZjdyx12114)
【分类号】：R742

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