丰富环境对HIBD新生大鼠海马CA1区BDNF及细胞凋亡的影响
发布时间：2018-08-26 21:02
【摘要】：目的：观察丰富环境对HIBD新生大鼠海马CA1区BDNF及细胞凋亡的影响,探讨丰富环境治疗HIBD的相关机制。方法：采用随机数字表法将7日龄Sprague-Dawley清洁级新生大鼠分为假手术组、模型组及丰富环境组,各组又分为术后14d、28d两个亚组(n=6)。参照Rice-Vannucci经典方法改良建立新生大鼠HIBD动物模型。模型组及假手术组置于标准环境中饲养,丰富环境组予以丰富环境干预治疗。术后14d、28d应用Morris水迷宫试验检测大鼠学习记忆能力,免疫组化法检测大鼠海马CA1区BDNF蛋白的阳性表达情况,TUNEL法检测海马CA1区细胞凋亡情况。结果：1.各组大鼠学习记忆能力：在术后14d、28d时间点上,与假手术组相比,模型组逃避潜伏期延长(P0.05),穿越平台次数减少(P0.05),差异具有统计学意义；与模型组相比,丰富环境组逃避潜伏期缩短(P0.05),穿越平台次数增多(P0.05),差异具有统计学意义。2.各组大鼠BDNF蛋白阳性表达变化：在术后14d、28d时间点上,与假手术组相比,模型组大鼠海马CA1区可见BDNF蛋白阳性表达升高,差异具有统计学意义(P0.0 5)；与模型组相比,丰富环境组大鼠海马CA 1区BDNF蛋白阳性表达更加明显,差异具有统计学意义(P0.05)。3.各组大鼠凋亡细胞阳性表达变化：在术后14d、28 d时间点上,与假手术组相比,模型组凋亡细胞阳性表达升高,差异具有统计学意义(P0.05)；与模型组相比,丰富环境组凋亡细胞阳性表达显著下降,差异具有统计学意义(P0.05)。结论：1.丰富环境可以改善HIBD大鼠学习记忆的能力。2.丰富环境可以上调HIBD大鼠海马CA 1区BDNF蛋白表达,下调HIBD大鼠海马CA1区凋亡细胞阳性表达。
[Abstract]:Aim: to observe the effect of rich environment on BDNF and apoptosis in CA1 area of hippocampus of neonatal HIBD rats and to explore the mechanism of rich environment therapy on HIBD. Methods: the 7-day-old Sprague-Dawley clean newborn rats were randomly divided into three groups: sham operation group, model group and rich environment group. Each group was divided into two subgroups: 14 days after operation and 28 days after operation (nong6). The HIBD animal model of newborn rats was established by reference to the classical Rice-Vannucci method. Model group and sham operation group were fed in standard environment, rich environment group was given rich environment intervention therapy. Morris water maze test was used to detect the learning and memory ability of rats and the positive expression of BDNF protein in hippocampus CA1 area was detected by immunohistochemical method. Tunel method was used to detect apoptosis in CA1 area of hippocampus. The result is 1: 1. Learning and memory ability of rats in each group: at the time point of 14 days and 28 days after operation, compared with the sham operation group, the escape latency of model group was prolonged (P0.05), the number of crossing platform was decreased (P0.05), the difference was statistically significant; compared with model group, The escape latency of rich environment group was shortened (P0.05), the number of crossing platform increased (P0.05), the difference was statistically significant. 2. The expression of BDNF protein in the model group was significantly higher than that in the sham-operated group (P 0.05), and the expression of BDNF protein was significantly higher in the model group than in the sham operation group (P 0.05), and the expression of BDNF protein in the hippocampus of the model group was significantly higher than that in the sham operation group (P 0.05). The positive expression of BDNF protein in hippocampal CA 1 region was more obvious in the rich environment group than in the control group, and the difference was statistically significant (P0.05). 3. The positive expression of apoptotic cells in the model group was significantly higher than that in the sham-operated group at the 28th day after operation (P0.05); compared with the model group, the positive expression of apoptotic cells in the model group was significantly higher than that in the control group (P0.05). The positive expression of apoptotic cells decreased significantly in the rich environment group (P0.05). Conclusion 1. Rich environment can improve learning and memory ability of HIBD rats. 2. 2. Rich environment could up-regulate the expression of BDNF protein in CA 1 area of hippocampus of HIBD rats and down-regulate the expression of apoptotic cells in CA1 area of hippocampus of HIBD rats.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R742
本文编号：2206148
[Abstract]:Aim: to observe the effect of rich environment on BDNF and apoptosis in CA1 area of hippocampus of neonatal HIBD rats and to explore the mechanism of rich environment therapy on HIBD. Methods: the 7-day-old Sprague-Dawley clean newborn rats were randomly divided into three groups: sham operation group, model group and rich environment group. Each group was divided into two subgroups: 14 days after operation and 28 days after operation (nong6). The HIBD animal model of newborn rats was established by reference to the classical Rice-Vannucci method. Model group and sham operation group were fed in standard environment, rich environment group was given rich environment intervention therapy. Morris water maze test was used to detect the learning and memory ability of rats and the positive expression of BDNF protein in hippocampus CA1 area was detected by immunohistochemical method. Tunel method was used to detect apoptosis in CA1 area of hippocampus. The result is 1: 1. Learning and memory ability of rats in each group: at the time point of 14 days and 28 days after operation, compared with the sham operation group, the escape latency of model group was prolonged (P0.05), the number of crossing platform was decreased (P0.05), the difference was statistically significant; compared with model group, The escape latency of rich environment group was shortened (P0.05), the number of crossing platform increased (P0.05), the difference was statistically significant. 2. The expression of BDNF protein in the model group was significantly higher than that in the sham-operated group (P 0.05), and the expression of BDNF protein was significantly higher in the model group than in the sham operation group (P 0.05), and the expression of BDNF protein in the hippocampus of the model group was significantly higher than that in the sham operation group (P 0.05). The positive expression of BDNF protein in hippocampal CA 1 region was more obvious in the rich environment group than in the control group, and the difference was statistically significant (P0.05). 3. The positive expression of apoptotic cells in the model group was significantly higher than that in the sham-operated group at the 28th day after operation (P0.05); compared with the model group, the positive expression of apoptotic cells in the model group was significantly higher than that in the control group (P0.05). The positive expression of apoptotic cells decreased significantly in the rich environment group (P0.05). Conclusion 1. Rich environment can improve learning and memory ability of HIBD rats. 2. 2. Rich environment could up-regulate the expression of BDNF protein in CA 1 area of hippocampus of HIBD rats and down-regulate the expression of apoptotic cells in CA1 area of hippocampus of HIBD rats.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R742
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