14-3-3β蛋白对胶质瘤细胞生物学行为的影响

发布时间：2018-08-29 12:10

【摘要】：目的探讨14-3-3β蛋白对胶质瘤细胞系生物学行为的影响。方法应用分子生物学技术构建14-3-3β蛋白的真核表达质粒pc DNA 3.1(-)-14-3-3β及干扰质粒pc DNATM6.2-GW/Em GFPmiR-14-3-3β,应用MTT、集落形成、Transwell侵袭试验研究14-3-3β蛋白对人胶质瘤细胞株U251增殖及侵袭等生物学行为的影响。结果过表达14-3-3β能够明显促进U251细胞(人胶质瘤系)的侵袭和增殖过程;而应用干扰质粒抑制14-3-3β表达后,U251细胞的侵袭和增殖能力则受到显著抑制。结论 14-3-3β对于U251细胞(人胶质瘤系)蛋白的侵袭和增殖能力起到明确的促进作用,其在人类胶质瘤的发生和发展过程中可能也起到促癌作用。
[Abstract]:Objective to investigate the effect of 14-3-3 尾 protein on the biological behavior of glioma cell line. Methods the eukaryotic expression plasmid pc DNA 3.1 (-) -14-3-3 尾 and the interfering plasmid pc DNATM6.2-GW/Em GFPmiR-14-3-3 尾 of 14-3-3 尾 protein were constructed by molecular biology technique. The effects of 14-3-3 尾 protein on the proliferation and invasion of human glioma cell line U251 were studied by MTT, colony forming transwell invasion test. Results overexpression of 14-3-3 尾 could significantly promote the invasion and proliferation of U251 cells (human glioma cells), while the ability of invasion and proliferation of U251 cells was significantly inhibited by using interference plasmids to inhibit the expression of 14-3-3 尾. Conclusion 14-3-3 尾 can promote the invasion and proliferation of U251 cell line (human glioma cell line), and it may also play a role in the carcinogenesis and development of human glioma.
【作者单位】：广州军区广州总医院神经外科;广东省第二人民医院中心实验室;
【基金】：广东省科技计划项目(编号:2013B021800048)
【分类号】：R739.41

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