缺血再灌注损伤大鼠患侧骨骼肌形态学及Atrogin-1、MuRF-1mRNA表达的变化
发布时间：2018-09-04 13:57
【摘要】：目的通过制作脑缺血再灌注损伤大鼠模型,观察大鼠脑缺血再灌住损伤早期患侧骨骼形态学变化及Atrogin-1和MuRF-1mRNA表达水平的变化。探讨脑缺血再灌注损伤大鼠患侧骨骼肌早期萎缩的发生机制。 方法60只雄性wistar大鼠,10只为假手术对照组(A组),其余50只采用Longa线栓法建立大鼠大脑中动脉栓塞模型(MCAO),其中30只造模成功大鼠随机分为B、C、D组,每组10只。B组为造模成功后1天组, C组为造模成功后4天组, D组为造模成功后7天组。应用Bederson评分评价动物的神经损伤后的恢复情况；分别获取A、B、C、D四组右侧肱二头肌,对各组通过HE染色检测肌纤维横截面积；通过荧光定量RT-PCR观察大鼠患侧骨骼肌中atrogin-1和MuRF-1mRNA表达的变化。 结果B、C、D组大鼠Bederson评分明显高于对照组A组(p0.05)；B、C、D组大鼠间Bederson评分无统计学差异(p0.05)。患侧骨骼肌HE染色显示A、B、C三组肌纤维横截面积两两比较,差异无统计学意义(p0.05)；D组大鼠患侧骨骼肌肌纤维横截面积分别与其余三组相比,差异均有统计学意义(p0.05)。B、C、D组大鼠患侧Atrogin-1和MuRF-1mRNA的表达水平分别与对照组相比,差异均有统计学意义(p0.05)；B组与C组相比较无显著差异(p0.05)；D组大鼠分别与B、C组相比,差异均有统计学意义(p0.05)。 结论脑缺血再灌注损伤大鼠早期患侧骨骼肌形态学可能已经发生变化,Atrogin-1和MuRF-1mRNA在脑缺血再灌注损伤大鼠早期骨骼肌中表达,脑缺血再灌注损伤大鼠早期骨骼肌中泛素连接酶蛋白降解通路可能已被激活。
[Abstract]:Objective to observe the changes of bone morphology and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats. To investigate the mechanism of early atrophy of skeletal muscle in rats with cerebral ischemia reperfusion injury. Methods 60 male wistar rats (n = 10) were used as sham-operated control group (group A). The other 50 rats were randomly divided into two groups: group A (n = 30), (MCAO), model of middle cerebral artery embolization (MCAO) in rats were established by Longa thread embolization, and 30 rats were randomly divided into two groups: group B (n = 30). There were 10 rats in each group. Group B was one day after successful modeling, group C was 4 days after successful modeling, and group D was 7 days after successful modeling. Bederson score was used to evaluate the recovery of nerve injury in animals, the right biceps brachii muscles of four groups were obtained respectively, and the cross sectional area of muscle fibers was detected by HE staining in each group. The changes of atrogin-1 and MuRF-1mRNA expression in skeletal muscle of the affected side of rats were observed by fluorescence quantitative RT-PCR. Results there was no significant difference in the Bederson score between the two groups compared with the control group A (p0.05) and the control group (p0.05). There was no significant difference in the Bederson score between the two groups (p0.05). HE staining of the affected skeletal muscle showed that the cross sectional area of muscle fibers in the three groups was not significantly different from that in the other three groups, and there was no significant difference in the cross sectional area of skeletal muscle between the D group and the other three groups. The expression levels of Atrogin-1 and MuRF-1mRNA in the affected side of rats in group B were significantly higher than those in group C (p0.05). There was no significant difference between group B and group C (p0.05), and there was no significant difference between group D and group C in the expression of Atrogin-1 and MuRF-1mRNA. The difference was statistically significant (p0.05). Conclusion the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury may have been changed in the skeletal muscle of rats with cerebral ischemia-reperfusion injury, and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats may have been changed. The proteolysis pathway of ubiquitin ligase in skeletal muscle may have been activated in the early stage of cerebral ischemia reperfusion injury in rats.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3
本文编号：2222308
[Abstract]:Objective to observe the changes of bone morphology and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats. To investigate the mechanism of early atrophy of skeletal muscle in rats with cerebral ischemia reperfusion injury. Methods 60 male wistar rats (n = 10) were used as sham-operated control group (group A). The other 50 rats were randomly divided into two groups: group A (n = 30), (MCAO), model of middle cerebral artery embolization (MCAO) in rats were established by Longa thread embolization, and 30 rats were randomly divided into two groups: group B (n = 30). There were 10 rats in each group. Group B was one day after successful modeling, group C was 4 days after successful modeling, and group D was 7 days after successful modeling. Bederson score was used to evaluate the recovery of nerve injury in animals, the right biceps brachii muscles of four groups were obtained respectively, and the cross sectional area of muscle fibers was detected by HE staining in each group. The changes of atrogin-1 and MuRF-1mRNA expression in skeletal muscle of the affected side of rats were observed by fluorescence quantitative RT-PCR. Results there was no significant difference in the Bederson score between the two groups compared with the control group A (p0.05) and the control group (p0.05). There was no significant difference in the Bederson score between the two groups (p0.05). HE staining of the affected skeletal muscle showed that the cross sectional area of muscle fibers in the three groups was not significantly different from that in the other three groups, and there was no significant difference in the cross sectional area of skeletal muscle between the D group and the other three groups. The expression levels of Atrogin-1 and MuRF-1mRNA in the affected side of rats in group B were significantly higher than those in group C (p0.05). There was no significant difference between group B and group C (p0.05), and there was no significant difference between group D and group C in the expression of Atrogin-1 and MuRF-1mRNA. The difference was statistically significant (p0.05). Conclusion the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury may have been changed in the skeletal muscle of rats with cerebral ischemia-reperfusion injury, and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats may have been changed. The proteolysis pathway of ubiquitin ligase in skeletal muscle may have been activated in the early stage of cerebral ischemia reperfusion injury in rats.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.3
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